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植物研究 ›› 2011, Vol. 31 ›› Issue (4): 397-402.doi: 10.7525/j.issn.1673-5102.2011.04.004

• 论文 • 上一篇    下一篇

大豆中β-伴大豆球蛋白提纯方法的优化

司晓霞1;王志2;霍斐1;史通麟3;付月君3;田怀东1*   

  1. 1.山西大学生命科学学院,太原 030006;2.山西省农作物品种资源研究所,太原 030031;3.山西大学生物技术研究所,太原 030006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-07-20 发布日期:2011-07-20
  • 通讯作者: 田怀东
  • 基金资助:
     

Optimization of Method for Extraction and Isolation of β-conglycinin from Soybean Seed

SI Xiao-Xia;WANG Zhi;HUO Fei;SHI Tong-Lin;FU Yue-Jun;TIAN Huai-Dong*   

  1. 1.College of Life Science,Shanxi University,Taiyuan 030006;2.Shanxi Institute of Crop Germplasm Resources,Taiyuan 030031;3.Institute of Biotechnology,Shanxi University,Taiyuan 030006
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20
  • Contact: TIAN Huai-Dong
  • Supported by:
     

摘要: β-伴大豆球蛋白(7S)是大豆种子储藏蛋白质的主要成分之一,对大豆蛋白质的营养品质有着重要影响。然而,如何提高该蛋白的纯度一直是影响其表征鉴定的一个主要问题。在本研究中,我们在Liu等报道方法的基础上,通过两个关键提取环节的条件优化,使β-伴大豆球蛋白的纯度得到了明显提高。一是在11S球蛋白去除之前提高所加还原剂SBS的浓度,分析SBS浓度提高对β-伴大豆球蛋白提取纯度的影响,将SBS的最适浓度确定为0.03 mmol·L-1;二是在β-伴大豆球蛋白析出之前,通过加水析出/离心法去除一次混杂的11S球蛋白。这两个环节的条件优化最终使得β-伴大豆球蛋白的提取纯度达到98%。本研究结果对大豆蛋白质遗传育种研究中β伴大豆球蛋白的表征鉴定具有重要意义。

关键词: 大豆, &beta, -伴大豆球蛋白, 提纯, 优化

Abstract: β-conglycinin, one of the major storage proteins in soybean seeds, greatly influences the quality of soybean seed proteins. However, increasing the purity of the protein has always been one main problem affecting its characterization and identification. In this study, the purity of β-conglycinin was obviously increased by performing condition optimization of the two key links in the process of extraction and isolation of the protein, based on the method reported by Liu et al. Firstly, SBS at increased concentrations was added to the protein solution before the removing of glycinin (11S), the effect of concentration increase on the purity of β-conglycinin was analyzed, and the optimal concentration was determined as 0.03 mmol·L-1; Secondly, the mixed glycinin was removed once more by the water precipitation/centrifugation method before precipitating β-conglycinin. The purity of β-conglycinin finally increased to 98% after optimization. The results have important significance for the characterization and identification of β-conglycinin in the genetic research and breeding for soybean proteins.

Key words: soybean, &beta, -conglycinin, extraction and isolation, optimization

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