花梨木叶中鹰嘴豆芽素A的提取工艺研究

doi:10.7525/j.issn.1673-5102.2011.03.021

植物研究 ›› 2011, Vol. 31 ›› Issue (3): 367-370.doi: 10.7525/j.issn.1673-5102.2011.03.021

花梨木叶中鹰嘴豆芽素A的提取工艺研究

王芳^1,2;张东阳^1,2;马飞跃^1,2;盖庆岩^1,2;焦骄^1,2;付玉杰^1,2*

1.东北林业大学森林植物生态学教育部重点实验室，哈尔滨　150040；2.东北林业大学林业生物制剂教育部工程研究中心，哈尔滨　150040

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
通讯作者: 付玉杰
基金资助:

The Extraction Process of Biochanin A from Dalbergia odorifera T. Chen Leaves

WANG Fang;ZHANG Dong-Yang;MA Fei-Yue;GAI Qing-Yan;JIAO Jiao;FU Yu-Jie;*

1.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Haerbin 150040；2.Engineering Research Center of Forest Bioprepration,Ministry of Education,Northeast University,Haerbin 150040

Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
Contact: FU Yu-Jie
Supported by:

摘要/Abstract

摘要： 以花梨木的可再生资源—叶子为原料，利用微波辅助酶提取技术进行提取，在单因素试验的基础上对提取条件进行了考察，根据BBD(Box-Behnken design)实验设计原理，采用3因素3水平的响应面分析法，以花梨木叶子中主要异黄酮鹰嘴豆芽素A(biochanin A)为指标，对提取过程进行优化，得到最佳工艺参数为：提取时间15 min，微波辐射功率300 W，提取温度34℃，pH值5.2，酶的加入量3.5 mg·g^-1。在最佳提取条件下，鹰嘴豆芽素A的提取率可达1.579 mg·g^-1。

关键词: 花梨木叶, 鹰嘴豆芽素A, 微波辅助酶提取, Box-Behnken设计

Abstract: The preliminary experiments and a three-variable, three-level Box-Behnken design were performed to study the microwave-assisted enzymatic extraction of biochanin A from Dalbergia odorifera T. Chen leaves. The optimum parameters were: extraction time 15 min, irradiation power 300 W, irradiation temperature 34℃, pH 5.2, amount of cellulase 3.5 mg·g^-1. Under the optimal conditions, the average extraction yield of biochanin A was 1.579 mg·g^-1.

Key words: Dalbergia odorifera T. Chen leaves, Biochanin A, microwave-assisted enzymatic extraction, Box-Behnken design

中图分类号:

S567

王芳;张东阳;马飞跃;盖庆岩;焦骄;付玉杰;*. 花梨木叶中鹰嘴豆芽素A的提取工艺研究[J]. 植物研究, 2011, 31(3): 367-370.

WANG Fang;ZHANG Dong-Yang;MA Fei-Yue;GAI Qing-Yan;JIAO Jiao;FU Yu-Jie;*. The Extraction Process of Biochanin A from Dalbergia odorifera T. Chen Leaves[J]. Bulletin of Botanical Research, 2011, 31(3): 367-370.

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花梨木叶中鹰嘴豆芽素A的提取工艺研究

The Extraction Process of Biochanin A from Dalbergia odorifera T. Chen Leaves

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