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植物研究 ›› 2010, Vol. 30 ›› Issue (6): 685-691.doi: 10.7525/j.issn.1673-5102.2010.06.011

• 论文 • 上一篇    下一篇

镉胁迫对矮牵牛种子萌发、幼苗生长及抗氧化酶活性的影响

范庆1;吕秀军2;杨柳1;杨颖丽1*   

  1. 1.西北师范大学生命科学学院,兰州 730070;2.甘肃省卫生学校,兰州 730000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-11-20 发布日期:2010-11-20
  • 通讯作者: 杨颖丽
  • 基金资助:
     

Effects of Cd Stress on the Seed Germination,Seedling Growth and Activities of Antioxidant Enzymes in Petunia hybrida

FAN Qing;LÜXiu-Jun;YANG Liu;YANG Ying-Li*   

  1. 1.College of Life Science,Northwest Normal University,Lanzhou 730070;2.Health School of Gansu Province,Lanzhou 730000
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20
  • Contact: YANG Ying-Li
  • Supported by:
     

摘要: 采用营养液培养的方法研究了不同浓度镉Cd(0、1、2和4 mmol·L-1)处理对矮牵牛(Petunia hybrida)种子萌发、幼苗生长及生理特性的影响。结果发现:与对照相比,较低浓度(1 mmol·L-1)Cd处理下矮牵牛种子萌发和幼苗生长均显著被抑制,当Cd浓度增至4 mmol·L-1时完全抑制了矮牵牛种子的萌发。Cd胁迫在低浓度时激活矮牵牛幼苗叶片过氧化物酶(POD)、过氧化氢酶(CAT)活性,而高浓度产生抑制效应;抗坏血酸过氧化物酶(APX)和超氧化物歧化酶(SOD)活性与对照相比均显著升高,丙二醛(MDA)含量无显著变化。不同浓度的乙二胺四乙酸(EDTA)的加入使4 mmol·L-1 Cd胁迫下矮牵牛幼苗叶片POD和APX活性增加,但不影响SOD和CAT活性;此外,6 mmol·L-1 EDTA使Cd胁迫下矮牵牛幼苗叶片中MDA含量增加。结果表明,Cd胁迫抑制矮牵牛种子萌发和幼苗生长,却使幼苗叶片POD、CAT和APX活性增加,EDTA的加入能够进一步提高Cd胁迫幼苗POD和APX活性。因此,矮牵牛幼苗在Cd胁迫下具有较强的抗氧化损伤的能力。

关键词: 抗氧化酶, 矮牵牛, 乙二胺四乙酸, 种子萌发, 镉胁迫

Abstract: Effects of different cadmium (Cd) concentrations (0, 1, 2 and 4 mmol·L-1) on the seed germination, seedling growth and physiological property of Petunia hybrida were studied. In comparison with the control, seed germination and seeding growth exhibited a significant decrease at low level of Cd2+ concentration(1 mmol·L-1), and these factors were all completely restrained at high concentration(4 mmol·L-1). Compared to the control, the activities of peroxidase (POD) and catalase (CAT) were enhanced in P.hybrida seedings exposed to low Cd concentrations but inhibited at higher concentrations; the activities of ascorbate peroxidase (APX) and superoxide dismutase (SOD) could be increased in response to different Cd concentrations, no significant change in the malonaldehyde (MDA) content was observed. Ethylene-aminetetraacetic acid (EDTA) could stimulate the activities of POD and CAT in P.hybrida seedlings exposed to 4 mmol·L-1 Cd treatment, and MDA content was also enhanced when seedlings were treated with 4 mmol·L-1 Cd in the presence of 6 mmol·L-1 EDTA. In conclusion, these results indicate that Cd stress significantly inhibit P.hybrida seed germination and seedling growth, but stimulate the activities of POD, CAT and APX in the leaves of seedlings; the application of EDTA further enhance the activities of POD and APX in Cd-treated P.hybrida seedlings. Therefore, higher activities of antioxidative enzymes may protect P.hybrida seedlings against Cd-induced oxidative change.

Key words: antioxidant enzymes, Petunia hybrida, Ethylene-aminetetraacetic acid, seed germination, cadmium stress

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