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植物研究 ›› 2010, Vol. 30 ›› Issue (3): 294-298.doi: 10.7525/j.issn.1673-5102.2010.03.008

• 论文 • 上一篇    下一篇

外源过氧化氢对小麦水分胁迫伤害的防护作用研究

邱宗波1;孙立1;李金亭1;魏改改1;岳明2   

  1. 1.河南师范大学生命科学学院,新乡 453007;2.西北大学生命科学学院,西安 710069
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:
     

Protecting Effect of Exogenous Hydrogen Peroxide on Wheat Seedlings Damage by Water Stress

QIU Zong-Bo;SUN Li;LI Jin-Ting;WEI Gai-Gai;YUE Ming   

  1. 1.College of Life Science,Henan Normal University,Xinxiang 453007;2.College of Life Science,Northwest University,Xi’an 710069
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Supported by:
     

摘要: 采用营养液水培的方法,研究外源过氧化氢(H2O2)对10% PEG6000(聚乙二醇)水分胁迫引起小麦幼苗脂质过氧化伤害的防护作用。结果表明,0.05 μmol·L-1 H2O2能够缓解水分胁迫对小麦幼苗生长的抑制效应,使幼苗株高、根长和单株干重显著增加,并提高受旱小麦叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性及谷胱甘肽和抗坏血酸的含量,降低丙二醛(MDA)含量和超氧自由基(O2-·)产生速率。说明外源H2O2可抑制水分胁迫引起小麦幼苗脂质过氧化作用,增强小麦的抗旱性。

关键词: 过氧化氢, 小麦, 水分胁迫, 脂质过氧化

Abstract: The study was conducted in nutrient solution to investigate the protecting effects of exogenous hydrogen peroxide (H2O2) on the wheat seedlings damage by water stress. The results showed that exogenous 0.05 μmol·L-1 H2O2 significantly alleviated the injury to seedlings and markedly increased the seedlings plant height, root length, dry weight, the contents of GSH, AsA and the activities of SOD, POD and CAT. However, exogenous 0.05 μmol·L-1 H2O2 markedly decreased the MDA concentration and the production rate of O2 in wheat seedling leaves under water stress. The results suggest that the treatment of H2O2 could restrain wheat seedlings lipid peroxidation by water stress and enhance anti-drought property of wheat seedlings.

Key words: H2O2, wheat, water stress, lipid peroxidation

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