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植物研究 ›› 2010, Vol. 30 ›› Issue (2): 197-201.doi: 10.7525/j.issn.1673-5102.2010.02.013

• 论文 • 上一篇    下一篇

八倍体小偃麦与天蓝偃麦草杂交F1染色体组构型

闫小丹;张延明;李集临*   

  1. 哈尔滨师范大学,哈尔滨 150025
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 李集临
  • 基金资助:
     

Elytrigia Genome Structure of Hybrid Octoploid Trititrigia and Agropyron glaucum

YAN Xiao-Dan;ZHANG Yan-Ming;LI Ji-Lin*   

  1. Harbin Normal University,Harbin 150025
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: LI Ji-Lin
  • Supported by:
     

摘要: 首次获得麦草8号、麦草9号、远中2号八倍体小偃麦与天蓝偃麦草的属间杂种,杂交当代结实率为31.49%,39.28%和10.41%。杂种F1表现为两亲的中间型,植株高大、繁茂,穗长20~30 cm,小穗数25~30个,多年生,抗寒,在哈尔滨冬季无覆盖条件下可安全过冬。对F1植株进行减数分裂行为观察,结果发现,染色体配对不正常,单价体频率高,出现多价体。杂种F1减数分裂中期I染色体配对构型分别为:9.5Ⅰ+16.98Ⅱ+0.27Ⅲ、13.6Ⅰ+14.01Ⅱ+0.87Ⅲ、11.2Ⅰ+16.8Ⅱ+0.08Ⅲ。二价体数变动在13~18间、单价体数变动在11~17间、多价体变动在0.08~0.87间,二价体多数是棒状二价体,推测两亲有一对部分同源关系染色体组,其余为非同源染色体组,但有的染色体间有部分同源关系。小麦5B染色体上Ph基因可能受到E组染色体的抑制。

关键词: 八倍体小偃麦, 天蓝偃麦草, 细胞遗传学, 减数分裂

Abstract: The intergeneric hybrid was obtained for the first time among Maicao 8th, Maicao 9th, Form 2nd of Octoploid Trititrigia and Agropyron glaucum. The seed-setting rate of crossing generation reached 31.49%, 39.28% and 10.41% respectively. Interstitial type of parents was showed on F_1 hybrid, the plants were very big and flourish, its ear length ranged from 20 cm to 30 cm, the number of spikelet from 25 to 30. The hybrid could survived without coverage during the winter in Harbin, because it was perennial plants and could resist the cold. After scrutinizing the meiosis of pollen mother cells in F_1 hybrids, the results were concluded as follows: the chromosomes were paired unusually, with high rate of univalent and appearing multibivalent. At F_1 PMC MI, the chromosome configurations were 9.5Ⅰ+16.98Ⅱ+0.27Ⅲ, 13.6Ⅰ+14.01Ⅱ+0.87Ⅲ and 11.2Ⅰ+16.8Ⅱ+0.08Ⅲ, respectively. The variable range of bivalent was 13 to 18, univalent was 11 to 17, and multivalent was 0.08 to 0.087, most of bivalents were bar shaped. We could speculate that part of homologous chromosome were in relationship and the others were non-homologous genomes, there was also relationship in some chromosome among homologous. Ph gene in chromosome of 5B of wheat may be inhibited by the chromosome E.

Key words: octoploid trititrigia, Agropyron glaucum, cytogenetics, meiosis

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