关键词: 京大戟, 3-羟基-3-甲基戊二酰辅酶A还原酶, 基因克隆

Abstract: Based on total RNA extracted from Euphorbia pekinensis, a 458 bp fragment of hmgr was obtained using reverse transcription PCR strategy and two degenerated oligonucleotides. Sequence analysis revealed that the conserved fragments are 458 bp. At the same time, the three fragments named hmgr1,hmgr2 and hmgr3 were obtained, which were 98.03%、96.29% and 78.38% identification in nucleotide acid compared with hmgr in E.pekinensis reported previously, and 98.68％, 96.71% and 85.53% identification in corresponding amino acid. The three fragments may be new members of hmgr gene family. Sequencing analysis also showed that the three framents had high identity with hmgr from other plants. Deduced amino acid sequence were also analyzed by PROSITE, ClustalX and Phylogenic tree, and data indicated that hmgr3 were different from hmgr1, hmgr2 and hmgr in E.pekinensis reported previously.

Key words: Euphorbia pekinensis Rupr, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGR), gene cloning