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植物研究 ›› 2008, Vol. 28 ›› Issue (5): 568-573.doi: 10.7525/j.issn.1673-5102.2008.05.014

• 论文 • 上一篇    下一篇

珍稀濒危植物长叶红砂种群遗传多样性的ISSR分析

张颖娟1,2;王玉山1   

  1. (1.内蒙古师范大学生命科学与技术学院,呼和浩特 010022) (2.内蒙古农业大学生态环境学院,呼和浩特 010019)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-09-20 发布日期:2008-09-20
  • 通讯作者: 张颖娟
  • 基金资助:
     

Analysis on the Population Genetic Diversity of Endangered Shrub Reaumuria trigyna by ISSR Markers

ZHANG Ying-Juan;WANG Yu-Shan   

  1. (1.Life Science and Technology College of Inner Mongolia Normal University,Hohhot 010022) (2.Ecology and Environmental College of Inner Mongolia Agricultural University,Hohhot 010019)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20
  • Contact: ZHANG Ying-Juan
  • Supported by:
     

摘要: 采用ISSR分子标记技术,对濒危小灌木长叶红砂(Reaumuria trigyna) 集中分布的5个种群的遗传多样性水平和遗传结构进行了研究。14条引物共检测到114个位点,其中99个为多态位点,多态位点比率为86.84%,长叶红砂种群具有较高的遗传多样性。物种水平上Shannon多样性指数(I)为0.468 8,Nei基因多样性指数(H)为0.308 4;种群水平上,多态位点比率P为77.89%,I为0.410 6,H为0.260 9,基因分化系数Gst为0.106 9,揭示了长叶红砂种群遗传变异多存在于种群内,种群间的遗传分化较小,占10.69%。 基因流(Nm)为4.178 7>1,说明种群间的基因交流,防止了由于遗传漂变导致的遗传分化。聚类分析表明长叶红砂种群遗传距离与地理距离之间无显著的相关性。研究结果说明遗传多样性水平与物种本身特性和所处不同群落有关,濒危植物并不一定表现为遗传变异水平的降低。

关键词: ISSR, 长叶红砂, 遗传多样性, 遗传结构

Abstract: Genetic diversity and genetic structure of five Reaumuria trigyna populations was analyzed by ISSR(inter simple sequence repeat) markers. Using 14 primers,114 loci were observed,among them 99 were polymorphic.The proportion of polymorphic loci was 86.84%. R.trigyna was the higher genetic diversity than other endangered shrubs. At species level,Shannon index(I) was 0.468 8,Nei’s gene diversity index(H) was 0.308 4. The proportion of polymorphic loci was 77.89%,I=0.410 6,H=0.260 9 respectively at population level. The coefficient of gene differentiation(Gst=0.106 9) showed that there were more genetic variations within the population than among populations,genetic differentiation among populations were 10.69%. Gene flow(Nm) was 4.178 7>1,showed that gene interflow were among populations,genetic differentiation were prevented due to genetic drift. Cluster analysis showed that there was no direct correlation between genetic distance and geographic distance. The results indicated that genetic diversity was related to species traits and community. The endangered plants was not certainly displayed lower level of genetic variation.

Key words: ISSR, Reaumuria trigyna, genetic diversity, genetic structure

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