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植物研究 ›› 2008, Vol. 28 ›› Issue (2): 232-235.doi: 10.7525/j.issn.1673-5102.2008.02.021

• 论文 • 上一篇    下一篇

拟南芥RabGAP7基因对ABA反应的功能分析

陈金峰1,2;胡斌杰2   

  1. (1.河南大学生命科学学院,开封 475001) (2.开封大学化学工程学院生物化学实验室,开封 475004)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-03-20 发布日期:2008-03-20
  • 通讯作者: 陈金峰
  • 基金资助:
     

Founctional Analysis of Arabidopsis RabGAP7 in Response to ABA

CHEN Jin-Feng;HU Bin-Jie   

  1. (1.Henan University,College of Life Science,Kaifeng 475001) (2.The biochemistry lab of College of Chemical Engineering Kaifeng University,Kaifeng 475004)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-03-20 Published:2008-03-20
  • Contact: CHEN Jin-Feng
  • Supported by:
     

摘要: 酶母的GYP能够加速小G蛋白YPT内在的GTPase的活性,是因为其基因所编码的氨基酸序列中具有保守的TBC区域,拟南芥AtGAPs的氨基酸序列中也具有此保守区,但对于它们的生物学功能,特别对胁迫的反应却研究不多。我们鉴定得到了RabGAP7基因的纯合突变体。通过根伸长和失水实验发现,与野生型相比,突变体幼苗对ABA和脱水不敏感。另外,表达分析表明,该基因在转录水平上对GPA1和Rab7起到负调控作用。这些结果暗示着RabGAP7可能通过调节G蛋白来参与了ABA反应。

关键词: 拟南芥, Rab, RabGAP7, ABA

Abstract: In S. cerevisiae, GYP can accelerate the intrinsic GTPase activity of YPT via the TBC active domain. In Arabidopsis, proteins containing similar TBC domain were called AtGAPs, but the biological function of AtGAPs, especially in stress response were not well known. There we isolated and characterized an Arabidposis T-DNA insertion mutant of RabGAP7. Phenotype analaysis suggested that the mutant was less sensitive to ABA and dehydration. Also it was found that the level of Rab7 and GPA1 on mRNA were lower in the wild-type than in rabgap7 plants by RT-PCR. So we conclude the RabGAP7 is involved the ABA response by activating the special G proteins.

Key words: Arabidopsis, Rab, RabGAP7, ABA

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