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植物研究 ›› 2008, Vol. 28 ›› Issue (2): 211-215.doi: 10.7525/j.issn.1673-5102.2008.02.017

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黑龙江省蹄盖蕨科(Athyriaceae)植物的RAPD分析及其系统学意义

范亚文;张秀英;门晓宇   

  1. (哈尔滨师范大学生物学系,哈尔滨 150025)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-03-20 发布日期:2008-03-20
  • 通讯作者: 范亚文
  • 基金资助:
     

RAPD Analysis of Athyriaceae in Heilongjiang Province and Its Phylogenetic Significance

FAN Ya-Wen;ZHANG Xiu-Ying;MEN Xiao-Yu   

  1. (Department of Biology,Harbin Normal University,Harbin 150025)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-03-20 Published:2008-03-20
  • Contact: FAN Ya-Wen
  • Supported by:
     

摘要: 在形态分类研究的基础上,利用RAPD技术,对黑龙江省蹄盖蕨科植物6属9种共15个种群进行了遗传多样性分析,分析结果表明:(1)假冷蕨属应包括在蹄盖蕨属中(2)角蕨属与蹄盖蕨属,羽节蕨属与冷蕨属亲源关系较近(3)短肠蕨属为独立一属,与其它属亲源关系较远。根据黑龙江省蹄盖蕨科植物的DNA水平的研究结果,再结合其形态学特征,建议将黑龙江省蹄盖蕨科划分为3个亚科:冷蕨亚科(Cystopterioideae)包括2个属:冷蕨属(Cystopteris)和羽节蕨属(Gymnocarpium)。蹄盖蕨亚科(Athyrioideae)包括3个属:蹄盖蕨属(Athyrium)、假冷蕨属(Pseudocystopteris)和角蕨属(Cornopteris)。双盖蕨亚科(Diplazioideae)包括短肠蕨属(Allantodia)。

关键词: 蹄盖蕨科, 系统发育关系, RAPD

Abstract:

6 genus 9 species, 15 populations of Athyriaceae from Heilongjiang province were analyzed using random amplified polymorphic DNA (RAPD) marks. The results show that: (1)Pseudocystopteris might be placed into Athyrium. (2)Cornopteris has a close relationship with Athyrium, as Gymnocarpium with Cystopteris. (3)Allantodia is an independent genus which has relatively little relationships with other genus in Athyriaceae. According to morphological characteristics and DNA levels, it is suggested that Athyriaceae from Heilongjiang province may be better divided into 3 subfamily, i.e. Cystopterioideae, including Cystopteris and Gymnocarpium; Athyrioideae, including Athyrium, Pseudocystopteris and Cornopteris; Diplazioideae including Allantodia.

Key words: Athyriaceae, phylogenetic relationship, RAPD

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