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植物研究 ›› 2008, Vol. 28 ›› Issue (1): 59-63.doi: 10.7525/j.issn.1673-5102.2008.01.013

• 论文 • 上一篇    下一篇

峨眉山2种重楼属植物同域分化的分子遗传学及其形态学证据初探

张瑞1;王丽2;唐铭霞2;翁周2   

  1. 1.内江师范学院,内江 641112 2.四川大学生命科学学院,成都 610064
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-01-20 发布日期:2008-01-20
  • 通讯作者: 张瑞
  • 基金资助:
     

The Genetic and Morphological Evidence for Sympatric Speciation Pattern of Paris from Emei Mountain

ZHANG Rui;WANG Li;TANG Ming-Xia;WENG Zhou   

  1. 1.Neijiang Teacthers College, Neijiang 641112 2.Institute of Life science, Sichuan University, Chengdu 610064
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-20 Published:2008-01-20
  • Contact: ZHANG Rui
  • Supported by:
     

摘要: 对峨眉山2种重楼-小重楼(Paris polyphylla Smith var. minora S.F.Wang)、黒籽重楼(P. thibetica Franch)DNA进行了AFLP (扩增的限制性片段长度多态性)分析和形态上的差异分析。AFLP分析可知小重楼和黑籽重楼在DNA分子水平上表现出极为丰富的遗传多样性,每个种内个体间的相似性很大,但有明显种间的遗传差异。形态对比分析可知小重楼和黑籽重楼在花部性状上有明显的差异。AFLP和形态分析结果表明小重楼和黑籽重楼无论是在形态还是遗传上均有明显差异。它们之间已经有了明显的分化。

关键词: 峨眉山重楼属, 同域分化, 小重楼, 黑籽重楼, AFLP, 遗传分化, 形态差异

Abstract: Amplified Fragment Length Polymorphism (AFLP) was used to analyze the differentiation of 2 natural species (Paris polyphylla Smith var. minora S.F.Wang & P. thibetica Franch)of Paris of EMEI Mountain. AFLP analysis indicated that there were relatively high genetic comparability among the two species and high genetic differentiation of the P. thibetica Franch and P. polyphylla var. minora with in a geographical region. The flower of morphological diversity was detected among the two species of Paris in EMEI Mountain by morphological analysis.The results suggested that high genetic differentiation and morphological differentiation of the P. thibetica and P. polyphylla Smith var. minora in a geographical region.

Key words: Emei Mountain, Paris, sympatric speciation, P. thibetica Franch, P. polyphylla Smith var. minora, AFLP, genetic differentiation, morphological differentiation

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