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植物研究 ›› 2006, Vol. 26 ›› Issue (1): 84-87.doi: 10.7525/j.issn.1673-5102.2006.01.014

• 论文 • 上一篇    下一篇

一种适用范围广的总RNA提取方法

王玉成;张国栋;姜 静   

  1. 东北林业大学林学院,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-01-20 发布日期:2006-01-20
  • 通讯作者: 王玉成
  • 基金资助:
     

A widely applied total RNA extraction method

WANG Yu-Cheng;ZHANG Guo-Dong;JIANG Jing   

  1. College of Forestry, Northeast Forestry University, Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-01-20 Published:2006-01-20
  • Contact: WANG Yu-Cheng
  • Supported by:
     

摘要: 介绍一种RNA提取方法,该方法以SDS、氯仿和Tris苯酚为主要提取试剂,以LiCl和乙醇为RNA沉淀试剂。分别以柽柳(木本植物)、星星草(草本植物)、天牛(昆虫)、酿酒酵母和白腐菌(真菌)为RNA提取材料,用该方法成功地提取出了它们的总RNA。获得的RNA条带清晰,A260/A280 在1.8以上。通过对LiCl和乙醇沉淀RNA的效果分析表明,该方法可在10 min内完全沉淀RNA,同时也可以同时获得纯度较高的DNA。提取的RNA质量可满足cDNA文库构建,基因芯片探针标定和RT-PCR等对RNA质量要求较高的分子生物学操作,说明这是一种应用范围广的RNA提取方法。

关键词: RNA, 提取, 方法

Abstract:

A total RNA extraction method is introduced, the main extraction reagents used in this method are SDS, chloroform and Tris-phenol, and the reagents of RNA precipitation are LiCl and ethanol. The total RNAs were successfully extracted from Tamarix androssowii(woody plant), Puccinellia tenuiflora (herbage), Saperda populnea (insect), Saccharomyces cerevisiae(fungi) and White-rot fungi by using this method. The total RNA extracted by this method produced clear bands, and the ratios of A260/A280 were above 1.8. The analysis of RNA precipitation efficiency showed that total RNA could be deposited completely in 10 minutes by the cooperation of LiCl and ethanol. At the same time, high purity DNA also can be isolated by this method, the quality of extracted RNA can meet the need of some molecule biology work requiring high quality RNA, such as cDNA library construction, labeling probes for gene chip and RT-PCR. The above described shows this method for RNA isolation can be widely applied.

Key words: RNA, extraction, method

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