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植物研究 ›› 2005, Vol. 25 ›› Issue (2): 216-218.doi: 10.7525/j.issn.1673-5102.2005.02.023

• 论文 • 上一篇    下一篇

灵芝孢子几种破壁方法比较分析

薛艳华1,2, 史权1,2, 庞海河1, 韩梅1, 杨蕾1   

  1. 1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
    2. 佳木斯大学, 佳木斯 154001
  • 收稿日期:2003-12-07 出版日期:2005-06-15 发布日期:2016-06-14
  • 作者简介:薛艳华(1971-),女,博士生,讲师,主要从事医学、微生物学和植物药的研究开发。

Comparing analysis on several wall-cracking methods of Ganoderma lucidum spore

XUE Yan-Hua1,2, SH IQuan1,2, PANG Hai-He1, HAN Mei1, YANG Lei1   

  1. 1. Key Laboratory of Forest Plant Ecolory, Ministry of Education, Northeast Forestry University, Harbin 150040;
    2. Jiamus University, Jiamus 154001
  • Received:2003-12-07 Online:2005-06-15 Published:2016-06-14

摘要: 采用超临界破壁法、匀浆破壁法和超声破壁法对灵芝孢子进行了破壁试验研究,研究显示,采用超临界高压静止破壁法可显著提高灵芝孢子的破壁率。匀浆破壁法和超声破壁法虽灵芝孢子破碎率较低,显微镜观察大部分孢子外形完整,但细胞内容物已几乎消失,达到提取孢子内容物的目的。

关键词: 灵芝孢子, 超临界高压静止破壁法, 匀浆破壁法

Abstract: Several methods on the wall-cracking of Ganoderma lucidum spore were carried on, mainly wall-cracking methods included supercritical wall-breaking, homogenate wall-cracking and ultrasonic wall-breaking. The results showed that adopting supercritical high-pressure steady state wall-cracking method could particularly improve the wall-crushing rate of Ganoderma lucidum spore. Although the wall-cracking rate of homogenate wall-cracking and ultrasonic wall-cracking was lower and most of the spores of Ganoderma lucidum were integrate in the microscope,the contents were nearly disappeared, attach to the purpose extracting effective constituentof spore inner.

Key words: Ganoderma lucidum spore, supercritical high-pressure steady state wall-cracking, homogenate wall-cracking