斯卑尔脱小麦1B染色体的显微分离及其DNA的PCR扩增

植物研究 ›› 2002, Vol. 22 ›› Issue (4): 487-491.

斯卑尔脱小麦1B染色体的显微分离及其DNA的PCR扩增

郭东林, 王同昌, 王宁, 徐淑红, 李集临

哈尔滨师范大学生物系, 哈尔滨 150080

收稿日期:2002-03-15 出版日期:2002-12-15 发布日期:2016-06-14
作者简介:郭东林(1973-),硕士,助教,主要从事分子遗传学研究。

MICRODISSECTION OF CHROMOSOME 1B OF TRITICUM SPELTA VAR. DUHAMELIANCUM AND AMPLIFICATION OF THE CHROMOSOME DNA

GUO Dong-lin, WANG Tong-cang, WANG Ning, XU Shu-hong, LI Ji-lin

Harbin Normal University, Harbin 150080

Received:2002-03-15 Online:2002-12-15 Published:2016-06-14

摘要/Abstract

摘要： 以斯卑尔脱小麦的一个变种及其近等基因系为材料,玻璃针法进行显微操作在减数分裂中期I分裂相中获得G型细胞质雄性不育的育性恢复基因Rf₃所在的1B染色体DNA。LA-PCR方法扩增DNA,斑点杂交的方法检测证明扩增产物来源于斯卑尔脱小麦基因组的1B染色体DNA。

关键词: 染色体显微操作, 小麦G型细胞质雄性不育, Rf₃基因, 1B染色体, LA-PCR

Abstract: Objective Gene Rf₃ originated from chromosome 1B of Triticum spelta var. Duhameliancum is a fertility-restorer gene for G-type cytoplasmic male sterility. Microdissected chromosome 1B by glass needles from cells in duration of meiotic metaphase. The DNA fragments was amplified by means of LA-PCR. By dot hybridization, the product was confirmed as the amplified DNA from chromosome 1B of T. spelta var. duhameliancum.

Key words: microdissection, G-type cytoplasmic male sterlity, gene Rf₃, Chromosome1B, LA-PCR

郭东林, 王同昌, 王宁, 徐淑红, 李集临. 斯卑尔脱小麦1B染色体的显微分离及其DNA的PCR扩增[J]. 植物研究, 2002, 22(4): 487-491.

GUO Dong-lin, WANG Tong-cang, WANG Ning, XU Shu-hong, LI Ji-lin. MICRODISSECTION OF CHROMOSOME 1B OF TRITICUM SPELTA VAR. DUHAMELIANCUM AND AMPLIFICATION OF THE CHROMOSOME DNA[J]. Bulletin of Botanical Research, 2002, 22(4): 487-491.