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植物研究 ›› 2022, Vol. 42 ›› Issue (1): 104-111.doi: 10.7525/j.issn.1673-5102.2022.01.011

• 研究报告 • 上一篇    下一篇

白桦BpPIN5基因启动子组织定位及外源激素应答分析

杨蕴力, 渠畅, 王阳, 刘桂丰, 姜静()   

  1. 林木遗传育种国家重点实验室,东北林业大学,哈尔滨 150040
  • 收稿日期:2020-10-08 出版日期:2022-01-20 发布日期:2021-12-30
  • 通讯作者: 姜静 E-mail:jiangjing1960@126.com
  • 作者简介:杨蕴力(1996—),女,硕士研究生,主要从事林木遗传育种研究。
  • 基金资助:
    林木遗传育种国家重点实验室创新项目(2020A01)

Tissue-specific Expression and Analysis of Exogenous Hormone Response of BpPIN5 Gene Promoter in Betula platyphylla

Yunli Yang, Chang Qu, Yang Wang, Guifeng Liu, Jing Jiang()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2020-10-08 Online:2022-01-20 Published:2021-12-30
  • Contact: Jing Jiang E-mail:jiangjing1960@126.com
  • About author:Yang Yunli(1996—),female,postgraduate,mainly engaged in tree genetics and breeding research.
  • Supported by:
    The Innovation Project of State Key Laboratory of Tree(2020A01)

摘要:

PIN家族蛋白作为IAA的极性输出载体,在植物胚胎发育、器官发育和向性生长,尤其是植物叶序、叶脉的形成及维管组织分化过程中起关键作用。为了明确白桦(Betula platyphyllaBpPIN5基因对外源激素的应答特性,实验以白桦全基因组DNA为参考,克隆获得BpPIN5基因的上游1 447 bp启动子序列,采用PLACE在线软件对该序列含有的顺式作用元件进行预测,结果表明,BpPIN5启动子序列含有生长素(IAA)、赤霉素(GA)、水杨酸(SA)、脱落酸(ABA)、茉莉酸甲酯(MeJA)、乙烯(ET)等不同类型的生长素响应元件。实验构建了pro-BpPIN5::GUS载体进行白桦转基因,GUS组化染色分析显示,BpPIN5启动子在白桦叶裂顶端、细叶脉及根中有转录活性。分别用IAA、GA、MeJA、SA及ABA激素处理转基因白桦,结果显示,BpPIN5启动子对上述5种激素在白桦第1叶片的裂叶边缘、第2叶的叶柄及根组织部位均有应答,且响应变化基本一致。研究结果为揭示白桦BpPIN5基因功能提供参考。

关键词: 白桦, 生长素运输载体, PINs, 激素应答

Abstract:

Being the polar carrier of IAA, PIN family proteins played a key role in plant embryonic development, organ development and tropic growth, especially in the formation of plant phyllodes, veins and vascular tissue differentiation.In order to identify the response characteristics BpPIN5 gene to exogenous hormones, the upstream 1447 bp sequence of BpPIN5 was cloned from the genome DNA of Betula platyphylla. The cis-acting elements in the sequence were predicted by PLACE online software. The results showed that the promoter sequence of BpPIN5 contained different types of auxin responsive elements such as auxin, gibberellin, Salicylic acid, abscisic acid, methyl jasmonate, ethylene respectively. Transgenic vector pro-BpPIN5::GUS to B. platyphylla was constructed. GUS histochemical staining analysis reflicted that BpPIN5 promoter had transcriptional activity in the tip of leaf crack, venule and root of B. platyphylla respectively. After transgenic Betula platyphylla was treated with IAA, GA, MeJA, SA and ABA respectively, the results showed that BpPIN5 promoter responded to the five hormones above at the crack edge of the first leaf, petiole and root tissue of the second leaf, and the response changes were basically the same. The results would help to clarify the function of BpPIN5 in B. platyphylla.

Key words: Betula platyphylla, auxin carrier, PINs, hormon response

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