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植物研究 ›› 2022, Vol. 42 ›› Issue (1): 29-38.doi: 10.7525/j.issn.1673-5102.2022.01.004

• 研究报告 • 上一篇    下一篇

毛竹扩展蛋白基因的鉴定及其表达分析

马霜1,2, 王博雅1, 曹颖1, 胡尚连1, 高志民2()   

  1. 1.西南科技大学生命科学与工程学院,绵阳 621000
    2.国家林业和草原局/北京竹藤科学与技术重点开放实验室,国际竹藤中心竹藤资源基因科学与基因产业化研究所,北京 100102
  • 收稿日期:2020-11-13 出版日期:2022-01-20 发布日期:2021-12-30
  • 通讯作者: 高志民 E-mail:gaozhimin@icbr.ac.cn
  • 作者简介:马霜(1995—),女,硕士研究生,主要从事林木遗传育种研究。
  • 基金资助:
    国际竹藤中心基本科研业务费专项资金项目(1632020004);国家自然科学基金(31971736)

Identification and Expression Analysis of Expansin Genes in Moso Bamboo (Phyllostachys edulis)

Shuang Ma1,2, Boya Wang1, Ying Cao1, Shanglian Hu1, Zhimin Gao2()   

  1. 1.College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621000
    2.State Forestry and Grassland Administration/Beijing Key Laboratory on the Science and Technology of Bamboo and Rattan,Institute of Gene Science and Industrialization for Bamboo and Rattan Resources,International Center for Bamboo and Rattan,Beijing 100102
  • Received:2020-11-13 Online:2022-01-20 Published:2021-12-30
  • Contact: Zhimin Gao E-mail:gaozhimin@icbr.ac.cn
  • About author:Ma Shuang(1995—),female,postgraduate,mainly engaged in tree genetics and breeding research.
  • Supported by:
    The special funds for fundamental scientific research on professional work supported by International Center for Bamboo and Rattan(1632020004);National Natural Science Foundation of China(31971736)

摘要:

为全面了解毛竹中扩展蛋白的分子特征和表达模式,本研究利用生物信息学方法在毛竹基因组中共鉴定出43个扩展蛋白基因家族成员,属于4个亚家族(EXPA、EXPB、EXLA和EXLB),分别包含18、17、7和1个成员,分布在37个Scaffold上。除PeEXPA1没有内含子和PeEXLB1含有11个内含子外,其它毛竹扩展蛋白基因的内含子为1~5个。毛竹扩展蛋白基因编码蛋白长度为91~508个氨基酸,所有的氨基酸都具有高频密码子,大部分蛋白为碱性亲水性蛋白。大部分毛竹扩展蛋白二级结构中β转角占比例最少,而β折叠占比例最大,各亚家族多数成员具有类似的三级结构。qRT-PCR结果表明,18个EXPA亚家族成员在不同组织表达存在明显差异,除PeEXPA2PeEXPA6外其它基因表达的最高值均出现在叶片中,表明它们可能在叶片生长过程中发挥着重要作用。

关键词: 毛竹, 扩展蛋白基因, 生物信息学, 表达分析

Abstract:

In order to fully understand the molecular characteristics and expression patterns of expansin genes in moso bamboo(Phyllostachys edulis), bioinformatics methods were used in this study,and a total of 43 members of the expansin gene family was identified from the genome of moso bamboo, and the members were divided into four subfamilies(EXPA, EXPB, EXLA and EXLB), which contained 18, 17, 7 and 1 members respectively, and distributed on 37 Scaffolds. Except that PeEXPA1 had no introns and PeEXLB1 had 11 introns, other expansin genes have 1 to 5 introns respectively. The amino acid length of the expansin was 91~508 aa respectively, all amino acids had high-frequency codons, and most of the expansins were basic and hydrophilic. Protein structure analysis showed that most of the expansins of moso bamboo accounted for the smallest proportion of β-turns and the largest proportion of β-sheets in the secondary structure. The tertiary structure of most members in each subfamily was similar. The qRT-PCR results of 18 EXPA subfamily members showed that their expression were significantly different in different tissues, and the highest expression values were found in leaves except PeEXPA2 and PeEXPA6, indicating that they might play an important role in the growth of leaves.

Key words: moso bamboo, expansin gene, bioinformatics, expression pattern analysis

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