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植物研究 ›› 2022, Vol. 42 ›› Issue (1): 21-28.doi: 10.7525/j.issn.1673-5102.2022.01.003

• 研究报告 • 上一篇    下一篇

碳源等因素对湿地松优良无性系胚性愈伤组织增殖的影响

高启阳, 黄宇龙, 郭文冰, 赵奋成, 刘阳()   

  1. 广东省林业科学研究院 广东省森林培育与保护利用重点实验室,广州 510520
  • 收稿日期:2020-09-11 出版日期:2022-01-20 发布日期:2021-12-30
  • 通讯作者: 刘阳 E-mail:yangliu90@sinogaf.cn
  • 作者简介:高启阳(1993—),女,硕士,主要从事松树体胚发生等方面的研究。
  • 基金资助:
    国家重点研发计划课题(2017YFD0600502)

Effects of Carbon Source and Other Factors on the Proliferation of Embryogenic Callus of Elite Clone of Pinus elliottii

Qiyang Gao, Yulong Huang, Wenbing Guo, Fencheng Zhao, Yang Liu()   

  1. Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangdong Academy of Forestry,Guangzhou 510520
  • Received:2020-09-11 Online:2022-01-20 Published:2021-12-30
  • Contact: Yang Liu E-mail:yangliu90@sinogaf.cn
  • About author:GAO Qi-Yang(1993—),female,master,research direction pine somatic embryogenesis.
  • Supported by:
    The National Key research and development program of China(2017YFD0600502)

摘要:

探究碳源等主要因素对湿地松胚性愈伤组织增殖的影响,为进一步提高其增殖效率提供技术方法和理论依据。利用优良无性系PEE3-13的胚性愈伤组织,研究不同种类及浓度的碳源、不同pH、有机氮源谷氨酰胺及其替代物丙谷二肽对其增殖的影响。结果发现培养基pH和碳源种类对其增殖的影响显著,实际pH范围为5.68~6.28时,最适宜胚性愈伤组织的生长,使用30 g·L-1白砂糖作为碳源时达到最高增殖率。丙谷二肽不能替代谷氨酰胺,添加450 mg·L-1谷氨酰胺时,胚性愈伤组织的增殖率最高、活力最好。当增殖培养基中使用 30 g·L-1白砂糖、添加450 mg·L-1的谷氨酰胺,实际pH为5.68~6.28时,最适宜该无性系胚性愈伤组织的增殖,增殖率最大可达到851.27%。

关键词: 湿地松, 胚性愈伤组织, 增殖培养, 碳源, pH, 谷氨酰胺

Abstract:

To explore the effects of carbon sources and other main factors on the proliferation of embryogenic callus in Pinus elliottii, and provide technical methods and theoretical basis for further improving proliferation efficiency. The embryogenic callus of the elite clone PEE3-13 was used, the effects of different types and concentrations of carbon sources, pH and the organic nitrogen sources, L-glutamine and its substitutes, L-alanine-L-glutamine on its proliferation were studied. The pH and the type of carbon source had significant effects on its proliferation. The autoclaved medium pH ranged from 5.68 to 6.28, it was the most suitable for the embryogenic callus growth. The maximum proliferation rate was achieved when using 30 g·L-1 white sugar was used as carbon source. When 450 mg·L-1 L-glutamine was added, the embryogenic callus had the highest proliferation rate and the best activity, however, L-alanine-L-glutamine could not replace L-glutamine. The optimal medium was 30 g·L-1 white sugar and 450 mg·L-1 L-glutamine and autoclaved pH was ranged from 5.68 to 6.28 and was most suitable for embryogenic callus proliferation, and the maximum proliferation rate was h 851.27%.

Key words: Pinus elliottii, embryogenic callus, proliferation culture, carbon source, pH, L-glutamine

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