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植物研究 ›› 2021, Vol. 41 ›› Issue (6): 870-877.doi: 10.7525/j.issn.1673-5102.2021.06.004

• 研究报告 • 上一篇    下一篇

外源H2S供体NaHS浸种对盐碱胁迫下裸燕麦种子萌发特性的影响

刘建新, 刘瑞瑞, 刘秀丽, 贾海燕, 卜婷, 李娜   

  1. 甘肃省高校陇东生物资源保护与利用省级重点实验室/陇东学院生命科学与技术学院,庆阳 745000
  • 收稿日期:2020-03-20 出版日期:2021-11-20 发布日期:2021-10-29
  • 作者简介:刘建新(1964—),男,教授,主要从事植物逆境生理生态研究。
  • 基金资助:
    国家自然科学基金(31960375);甘肃省自然科学基金(20JR5RA491)

Effect of Soaking Seeds with NaHS on Seed Germination Characteristics of Naked Oat under Saline-Alkali Stress

Jian-Xin LIU, Rui-Rui LIU, Xiu-Li LIU, Hai-Yan JIA, Ting BU, Na LI   

  1. University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province/College of Life Sciences and Technology,Longdong University,Qingyang 745000
  • Received:2020-03-20 Online:2021-11-20 Published:2021-10-29
  • About author:LIU Jian-Xin(1964—),male,professor,mainly engaged in plant stress physiological ecology.
  • Supported by:
    National Natural Science Foundation of China(31960375);Natural Science Foundation of Gansu Province(20JR5RA491)

摘要:

硫化氢(H2S)信号在作物种子萌发和逆境响应中发挥着重要作用。为探讨外源H2S供体NaHS浸种对盐碱胁迫下作物种子萌发的影响,以裸燕麦(Avena nude)为材料,用不同浓度NaHS(0、50、100、200和400 μmol·L-1)浸种8 h后分别在浓度为0、15、30、45和60 mmol·L-1的混合盐碱(NaCl∶Na2SO4∶Na2CO3∶NaHCO3摩尔比为12∶8∶1∶9)胁迫下进行种子萌发试验,分析其发芽率(Gp)、发芽指数(Gi)、活力指数(Vi)、平均发芽时间(MGT)、幼苗干重(SDW)和隶属函数综合评价值(D)的变化。结果表明:盐碱浓度和NaHS浓度及两者的交互作用对Gp、Gi、Vi、MGT、SDW和D值存在显著(P<0.05)或极显著(P<0.01)的影响。随着盐碱浓度提高,Gp、Gi、Vi、MGT、SDW和D值呈显著下降变化。与0 μmol·L-1 NaHS对照(CK)相比,50和100 μmol·L-1 NaHS不同程度提高了0 mmol·L-1盐碱胁迫下的Gp、Gi、Vi和D值;50 μmol·L-1 NaHS显著提高了30 mmol·L-1盐碱胁迫下的Gp;50和100 μmol·L-1 NaHS不同程度提高了15、30和45 mmol·L-1盐碱胁迫下的Gi;50 μmol·L-1 NaHS显著提高了30 mmol·L-1盐碱胁迫下的Vi;50和100 μmol·L-1 NaHS显著提高了45 mmol·L-1盐碱胁迫下的D值;其他处理的各发芽指标值与CK的差异不显著或显著降低。这些结果说明,适宜浓度NaHS浸种能够缓解盐碱胁迫对裸燕麦种子萌发的抑制,其中以50 μmol·L-1 NaHS缓解45 mmol·L-1以下浓度盐碱胁迫的效果最佳,200和400 μmol·L-1 NaHS加重盐碱胁迫对种子萌发的抑制,NaHS对高浓度(60 mmol·L-1)盐碱抑制裸燕麦种子萌发无缓解作用。

关键词: 硫化氢, 裸燕麦, 盐碱胁迫, 种子萌发

Abstract:

Hydrogen sulfide(H2S) signaling plays an important role in seed germination and stress response. To explore the influence of soaking seeds with sodium hydrosulfide(NaHS, a donor of H2S) on the seed germination of crops under saline-alkali stress, naked oat(Avena nude) seeds were selected as experimental material which were soaked with different concentration(0, 50, 100, 200 and 400 μmol·L-1) of NaHS for 8 h, and then germinated in different concentrations of mixed saline-alkaline(the mole ratio of NaCl∶Na2SO4∶Na2CO3∶NaHCO3 is 12∶8∶1∶9) solution(0, 15, 30, 45 and 60 mmol·L-1) in petri dishes in incubator. Changes in germination percentage(Gp), germination index(Gi), vigor index(Vi), mean germination time(MGT), dry weight of seedlings(SDW) and comprehensive evaluation value of membership function(D) were analyzed. The effects of different concentrations of saline-alkali and NaHS and their interaction on Gp, Gi, Vi, MGT, SDW and D were significant(P<0.05) or extremely significant(P<0.01). The Gp, Gi, Vi, MGT, SDW and D decreased significantly with the increase of saline-alkali concentration. Compared with 0 μmol·L-1 NaHS(CK), 50 and 100 μmol·L-1 NaHS increased the Gp, Gi, Vi and D under 0 mmol·L-1 saline-alkali stress to different degrees. The 50 μmol·L-1 NaHS significantly increased the Gp under 30 mmol·L-1 saline-alkali stress. The 50 and 100 μmol·L-1 NaHS increased Gi under 15, 30 and 45 mmol·L-1 saline-alkali stress to different degrees. The 50 μmol·L-1 NaHS significantly increased Vi under 30 mmol·L-1 saline-alkali stress. The 50 and 100 μmol·L-1 NaHS significantly increased D under 45 mmol·L-1 saline-alkali stress. However, the germination index values of other treatments were not significantly different or significantly reduced. These results suggest that soaking the seed with NaHS of appropriate concentration could relieve the inhibition of saline-alkali stress on seed germination of naked oats, optimal effects were reached at NaHS concentrations of 50 μmol·L-1 under saline-alkali stress at below 45 mmol·L-1, 200 and 400 mmol·L-1 NaHS aggravated the inhibition of salinity-alkali stress on seed germination, and NaHS had no palliative effect on the inhibition of seed germination of naked oat under high concentration of salinity-alkali stress(60 mmol·L-1).

Key words: hydrogen sulfide, naked oat, saline-alkali stress, seed germination

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