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植物研究 ›› 2021, Vol. 41 ›› Issue (5): 798-806.doi: 10.7525/j.issn.1673-5102.2021.05.018

• 研究报告 • 上一篇    

基于GC-MS分析东北红豆杉野生种与栽培种的代谢差异

刘龙桀1, 吴可心1, 刁云飞2, 李中跃1, 穆立蔷1()   

  1. 1.东北林业大学林学院,哈尔滨 150040
    2.黑龙江牡丹江森林生态系统定位研究站,哈尔滨 150081
  • 收稿日期:2021-03-20 出版日期:2021-09-20 发布日期:2021-07-05
  • 通讯作者: 穆立蔷 E-mail:mlq0417@163.com
  • 作者简介:刘龙桀(1993—),男,硕士研究生,主要从事资源植物保护与利用的研究。
  • 基金资助:
    国家科技基础资源调查专项(2019FY100500);东北红豆杉野外种群的更新扩散机制及保护策略(20189037)

Metabolic Differences Between Wild and Cultivated Species of Taxus cuspidata Based on GC-MS

Long-Jie LIU1, Ke-Xin WU1, Yun-Fei DIAO2, Zhong-Yue LI1, Li-Qiang MU1()   

  1. 1.Forestry College,Northeast Forestry University,Harbin 150040
    2.Mudingjiang Forest Ecosystem Research Station,Harbin 150081
  • Received:2021-03-20 Online:2021-09-20 Published:2021-07-05
  • Contact: Li-Qiang MU E-mail:mlq0417@163.com
  • About author:LIU Long-Jie(1993—),male,master graduate,mainly engaged in the protection and utilization of resource plants.
  • Supported by:
    Science and Technology Basic Resources Investigation Program of China(2019FY100500);Regeneration Diffusion Mechanism and Conservation Strategy of Taxus cuspidata wild population(20189037)

摘要:

为了探究东北红豆杉野生种和栽培种之间代谢物质的差异,对其进行非靶向代谢组学的分析。利用GC-MS技术以及多元统计分析对野生种和栽培种的树皮进行比较,发现两者代谢物质有差异,总共检测到45种显著差异代谢物,主要包括4种氨基酸、10种有机酸和一些糖醇等。其中有22个代谢物质表达上调,23个表达下调,通过KEGG进行通路分析发现其中有7条通路影响较显著,分别为硫代谢,精氨酸和脯氨酸代谢,磷酸戊糖途径,不饱和脂肪酸的生物合成,半乳糖代谢,丙氨酸、天冬氨酸和谷氨酸代谢,苯丙烷类生物合成。其中检测到的代谢物如氨基酸和有机酸在栽培种中有更高的表达,东北红豆杉野生种在抗逆性能上弱于栽培种。而糖类却在野生种中表达更高,东北红豆杉野生种拥有更好的糖代谢能力,来抵御低温胁迫,本实验为指导东北红豆杉野生种的保护和东北红豆杉的抗逆性机理的研究提供了理论依据。

关键词: 东北红豆杉, 代谢组学, GC-MS

Abstract:

In order to explore the differences of metabolites between wild and cultivated Taxus cuspidata, nontargeted metabolomics was analyzed. Using GC-MS and multivariate statistical analysis to compare the bark of wild species and cultivated species, the differences in their metabolites were found. A total of 45 significant metabolites were detected, including 4 amino acids, 10 organic acids and some sugar alcohols. Among them, 22 metabolites were up-regulated and the 23 metabolites were down-regulated. KEGG pathway analysis showed that seven pathways had significant effects, including sulfur metabolism, arginine and proline metabolism, pentose phosphate pathway, unsaturated fatty acid biosynthesis, galactose metabolism, alanine, aspartate and glutamic acid metabolism, phenylpropanoid biosynthesis. The metabolites such as amino acids and organic acids were highly expressed in cultivated T. cuspidata. The stress resistance of wild T. cuspidata was weaker than that of cultivated T. cuspidata. However, the expression of carbohydrate was higher in wild species. T. cuspidata wild species had better glucose metabolism ability to resist low temperature stress. The results provided a theoretical basis for the protection of T. cuspidata wild species and stress resistance mechanism of T. cuspidata.

Key words: Taxus cuspidata, metabonomics, GC-MS

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