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植物研究 ›› 2021, Vol. 41 ›› Issue (5): 753-759.doi: 10.7525/j.issn.1673-5102.2021.05.014

• 研究报告 • 上一篇    

红花龙胆组培快繁体系研究

钟世浚, 张碧东, 赖世婷, 刘华敏()   

  1. 重庆文理学院园林与生命科学学院,永川 402160
  • 收稿日期:2020-04-26 出版日期:2021-09-20 发布日期:2021-07-05
  • 通讯作者: 刘华敏 E-mail:liuhuanin@126.com
  • 作者简介:钟世浚(1999—),男,本科生,主要从事植物繁育方面的研究。
  • 基金资助:
    重庆市大学生创新训练项目;重庆市科技计划项目(cstc2019jcyj-msxmX0687)

Tissue Culture and Rapid Propagation System of Gentiana rhodantha

Shi-Jun ZHONG, Bi-Dong ZHANG, Shi-Ting LAI, Hua-Min LIU()   

  1. School of Garden and Life Sciences,Chongqing University of Arts and Sciences,Yongchuan 402160
  • Received:2020-04-26 Online:2021-09-20 Published:2021-07-05
  • Contact: Hua-Min LIU E-mail:liuhuanin@126.com
  • About author:ZHONG Shi-Jun(1999—),male,undergraduate,mainly research on the breeding of plants.
  • Supported by:
    College Students’ of Chongqing Innovative Entrepreneurial Training Plan Program;Chongqing province science and technology innovation project(cstc2019jcyj-msxmX0687)

摘要:

为建立红花龙胆组培快繁体系,对红花龙胆合适外植体、消毒方法、最佳增殖培养基、生根培养基及移栽驯化方法进行了研究。结果表明,带节茎段为红花龙胆组织培养的最佳外植体;5%的次氯酸钠消毒10 min,污染率为0,成活率达80%;芽诱导增殖最佳培养基为:MS+0.1 mg·L-1 IBA+2.5 mg·L-1 ZT,增殖系数为8.94;最佳生根培养基为:1/2MS+0.3 mg·L-1 IBA,生根率为100%;1∶1的珍珠岩∶草炭为最佳移栽基质,其移栽成活率达100%。为红花龙胆快速育苗与规模化种植提供技术参考。

关键词: 红花龙胆, 外植体, 组织培养

Abstract:

To establish the tissue culture system of Gentiana rhodantha, the proper explant, disinfecting methods, the best medium for seedling proliferation, for rooting and methods for transplanting were studied respectively. The results showed that the stems with node were the best explants, and the survival rate reached 80% with no contamination by disinfecting with 5% sodium hypochlorite for 10 min; the best medium for bud proliferation was: MS+0.1 mg·L-1 IBA+2.5 mg·L-1 ZT with a proliferation coefficient of 8.94, and the best rooting medium was: 1/2MS+0.3 mg·L-1 IBA with a rooting rate of 100%; the best transplant medium was perlite∶turf=1∶1 with a survival rate up to 100%. This study established a rapid propagation system, provided the technical support for the factory seedling and large-scale cultivation of G. rhodantha.

Key words: Gentiana rhodantha, polyploid, tissue culture

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