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植物研究 ›› 2021, Vol. 41 ›› Issue (5): 721-728.doi: 10.7525/j.issn.1673-5102.2021.05.010

• 研究报告 • 上一篇    

3种红豆杉茎段腋芽启动组培繁育技术研究

焦骄, 刘婧, 付玉杰, 王馨, 盖庆岩(), 鹿瑶, 徐晓杰, 王紫莹   

  1. 东北林业大学化学化工与资源利用学院,森林植物生态学教育部重点实验室,哈尔滨 150040
  • 收稿日期:2021-03-11 出版日期:2021-09-20 发布日期:2021-07-05
  • 通讯作者: 盖庆岩 E-mail:gqy_nefu@163.com
  • 作者简介:焦骄(1986—),男,副教授,主要从事植物离体细胞/组织/器官培养技术研究。
  • 基金资助:
    “十三五”国家重点研发计划项目(2017YFD0600205);国家林业和草原科技创新青年拔尖人才项目(2020132611)

In vitro Propagation of Stem Axillary Buds of Three Species of Taxus Using Tissue Culture

Jiao JIAO, Jing LIU, Yu-Jie FU, Xin WANG, Qing-Yan GAI(), Yao LU, Xiao-Jie XU, Zi-Ying WANG   

  1. College of Chemistry,Chemical Engineering and Resource Utilization,Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Received:2021-03-11 Online:2021-09-20 Published:2021-07-05
  • Contact: Qing-Yan GAI E-mail:gqy_nefu@163.com
  • About author:JIAO Jiao(1986—),male,associate professor,majoring in plant cell, tissue, and organ culture technology.
  • Supported by:
    National Key R&D Program of China(2017YFD0600205);National Forestry and Grassland Science and Technology Innovation Program for Top Talented Youths(2020132611)

摘要:

红豆杉属植物由于天然生长缓慢、繁育能力衰退和种群竞争力弱等客观因素以及人为过度开发利用和生境破坏等主观因素已濒临灭绝,因此对其种质资源的繁育保护已成为当前研究的热点。本研究以我国特有的3种红豆杉(东北红豆杉、南方红豆杉和曼地亚红豆杉)为研究对象,选取带有腋芽的茎段组织为外植体,系统考察了不同植物激素组合对于腋芽的启动、增殖及生根的影响,最终确定了MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA为腋芽启动培养基,MS+0.4 mg·L-1 6-BA+1.0 mg·L-1 NAA为不定芽增殖培养基,发现3种红豆杉在上述培养基中都能够有效启动和增殖;同时,优化确定了WPM+0.8 mg·L-1 NAA+0.2 mg·L-1 IBA培养基可促使东北红豆杉不定芽生根,且不添加任何植物激素的WPM培养基有利于生根苗的后续生长。本研究建立了一种简单有效的红豆杉体外繁育方法,为我国红豆杉资源的保护和可持续利用提供了有力的技术支持。

关键词: 红豆杉, 茎段腋芽, 繁育, 离体培养

Abstract:

Taxus species are imminent extinction due to objective factors such as slow growth speed, decreased breeding ability, and weak competition, as well as subjective factors including overexploitation and habitat destruction. Therefore, the protection of Taxu resources via breeding methods has become the focus of research. In this study, three Taxus species in China(Taxus cuspidataT. mairei, and T. media) were selected as materials, and the stem tissues with axillary buds were selected as explants, and the different phytohormone combinations on the initiation, proliferation and rooting of axillary buds were systematically investigated. MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA was finally determined as the optimal medium for axillary bud initiation, and MS+0.4 mg·L-1 6-BA+1.0 mg·L-1 NAA was the suitable medium for adventitious bud proliferation. It was found that all three Taxus species could effectively initiated and proliferate in the above-mentioned medium. Moreover, WPM+0.8 mg·L-1 NAA+0.2 mg·L-1 IBA medium was found to promote the rooting of adventitious buds of T. cuspidata, and the WPM medium without adding any plant hormones was beneficial for the subsequent growth of rooted seedlings. Overall, this study established a simple and effective method for in vitro propagation of Taxus species, which would provide the technical support for the protection and sustainable utilization of Taxus resources in China.

Key words: Taxus species, stem axillary bud, propagation, in vitro culture

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