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植物研究 ›› 2021, Vol. 41 ›› Issue (5): 684-689.doi: 10.7525/j.issn.1673-5102.2021.05.006

• 研究报告 • 上一篇    

橙花破布木染色体制片及核型分析

陈意兰1,2, 廖海民2, 王峥峰1, 黄向旭1, 刘东明1()   

  1. 1.中国科学院华南植物园,中国科学院南海生态环境工程创新研究院,广州 510650
    2.贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳 550025
  • 收稿日期:2021-04-01 出版日期:2021-09-20 发布日期:2021-07-05
  • 通讯作者: 刘东明 E-mail:Liudm@scbg.ac.cn
  • 作者简介:陈意兰(1992—),女,硕士研究生,主要从事植物资源评价与利用、特殊生境生态修复方面的研究。
  • 基金资助:
    中国科学院A类战略性先导科技专项(XDA13020500);国家自然科学基金(41571056);河北省交通运输厅科技攻关项目(QG2018-10)

Chromosome Preparation and Karyotype Analysis of Cordia subcordata

Yi-Lan CHEN1,2, Hai-Min LIAO2, Zheng-Feng WANG1, Xiang-Xun HUANG1, Dong-Ming LIU1()   

  1. 1.South China Botanical Garden,Innovation Academy of South China Sea Ecology and Environmental Engineering,Chinese Academy of Sciences,Guangzhou 510650
    2.Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering,College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025
  • Received:2021-04-01 Online:2021-09-20 Published:2021-07-05
  • Contact: Dong-Ming LIU E-mail:Liudm@scbg.ac.cn
  • About author:CHEN Yi-Lan(1992—),femal,master candidate,mainly engaged in evaluation and utilization of plant resources, ecological restoration of special habitats.
  • Supported by:
    the Strategic Priority Research Program of Chinese Academy of Sciences(XDA13020500);Natural Science Foundation of China(41571056);Science and Technology Breakthrough Project of Hebei Provincial Department of Transport(QG2018-10)

摘要:

为了解橙花破布木的遗传背景,以其根尖为试验材料,采用体细胞染色体常规制片法着重探索取材和预处理两个实验环节,选取染色体分散较好的细胞进行数目确定及核型分析。结果表明:(1)橙花破布木最佳取材时间为9:30~10:00和14:00~14:30,最佳预处理试剂及时间为饱和对二氯苯预处理2h;(2)橙花破布木染色体数目为32条,共16对染色体,为二倍体植物;核型公式为2n=2X=32=32m,核型属于“1A”型;染色体组绝对长度变化范围为0.38~0.69μm,相对长度(%)变化范围为4.48~8.24,相对长度组成为2n=2L+14M1+14M2+2S;核型不对称系数(As.K%)为58.40%。研究结果为破布木属植物染色体制片技术及核型分析提供参考,也可为橙花破布木基因进行染色体定位等细胞遗传学及表观遗传学研究奠定基础。

关键词: 橙花破布木, 染色体, 核型分析

Abstract:

In order to understand the genetic background of Cordia subcordata, we used the root tips as materials to investigate optimal sampling and pretreatment conditions, and the cells with better chromosome dispersion were selected for karyotype analysis. The results showed that:(1)The best sampling time of C. subcordata was from 9:30-10:00 am and from 14:00-14:30 pm and the optimal pretreatment was immersing the root tips in saturated p-dichlorobenzene for 2 hours; (2)C. subcordata was diploid, with 32 chromosomes, totally 16 pairs. The karyotype formula was 2n=2X=32=32m, and the karyotype was “1A”. The absolute length variation range of chromosome was 0.38-0.69 μm, and the relative length(%) varied from 4.48-8.24. The relative length composition was 2n=2L+14M1+14M2+2S. The karyotype asymmetry coefficient(As.K%) was 58.40%. These results would provide references for chromosome preparation and karyotype analysis, and laid a foundation for cytogenetic and epigenetic studies on C. subcordata.

Key words: Cordia subcordata, chromosome, karyotype analysis

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