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植物研究 ›› 2020, Vol. 40 ›› Issue (3): 447-457.doi: 10.7525/j.issn.1673-5102.2020.03.017

• 研究报告 • 上一篇    

红松查尔酮合成酶基因CHS密码子偏好性分析

李翔1, 范作义2, 王井源2, 王淇2, 李喜鹏2, 王德秋2, 孔令远2, 曹森林2, 孟庆刚2, 赵曦阳1   

  1. 1. 林木遗传育种国家重点实验室, 东北林业大学林学院, 哈尔滨 150040;
    2. 吉林省临江林业局, 临江 134600
  • 收稿日期:2019-08-25 发布日期:2020-05-29
  • 通讯作者: 赵曦阳,E-mail:zhaoxyphd@163.com E-mail:zhaoxyphd@163.com
  • 作者简介:李翔(1993-),男,博士研究生,主要从事红松遗传改良方面的研究。
  • 基金资助:
    红松种质资源遗传多样性评价项目(KJZXSA2019046)

Codon Usage Bias of Chalcone Synthase Gene CHS in Pinus koraiensis

LI Xiang1, FAN Zuo-Yi2, WANG Jing-Yuan2, WANG Qi2, LI Xi-Peng2, WANG De-Qiu2, KONG Ling-Yuan2, CAO Sen-Lin2, MENG Qing-Gang2, ZHAO Xi-Yang1   

  1. 1. State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040;
    2. Linjiang Forestry Bureau of Jilin Province, Linjiang 134600
  • Received:2019-08-25 Published:2020-05-29
  • Supported by:
    Genetic Diversity Evaluation Project of Pinus koraiensis Germplasm Resources(KJZXSA2019046)

摘要: 查尔酮合成酶(Chalcone synthase,CHS)广泛存在于植物体内,是花色素形成过程中一种重要的酶,可以进一步催化生成黄酮类化合物。本研究采用CodonW和EMBOSS在线软件对红松查尔酮合成酶基因CHS的密码子使用偏好性进行分析,并与北美乔松等其他24种植物的CHS基因以及模式植物基因组进行比较,对认识红松CHS基因的密码子使用偏好性,为选择适宜的表达系统奠定了一定的基础。研究结果表明:红松CHS基因编码区的有效密码子数(ENC)和GC含量分别为48.92和0.548,C+G含量高于A+T含量,密码子偏好以A/T结尾;多数植物CHS基因的G+C含量高于A+T含量,且密码子更偏好C/G结尾;聚类分析表明,红松与马尾松和赤松的密码子使用偏好性的相似性较高;密码子使用频率研究发现,红松CHS遗传转化与异源表达较优的受体可能是大肠杆菌和拟南芥。

关键词: 红松, 查尔酮合成酶, 密码子偏好性, 聚类分析

Abstract: Chalcone synthase(CHS) is widely found in plants and is a key enzyme in anthocyanin synthesis pathway. It can catalyze the binding of malonyl coenzyme A and coumaryl coenzyme A to generate flavonoids. CodonW and EMBOSS were used to analyze the codon usage bias of CHS gene in Pinus koraiensis. The CHS gene of P.koraiensis were compared with those of the other 24 plants and model plant genome, which laid a foundation for the selection of appropriate expression system. The effective codon number(ENC) and GC content of CHS gene coding region were 48.92 and 0.351, respectively. The content of C+G was higher than that of A+T, and the codon preferred to end with A/T. The G+C content of most plants CHS genes is higher than that of A+T, and the codon preferred to end with C/G. By cluster analysis, the codon usage bias of P.koraiensis was similar to P.massoniana and P.densiflora pdchsx. In addition, it was found that Arabidopsis thaliana and Escherichia coli might be ideal receptor systems for CHS genetic transformation and heterogeneous expression.

Key words: Pinus koraiensis, chalcone synthase, codon usage bias, cluster analysis

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