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植物研究 ›› 2020, Vol. 40 ›› Issue (3): 424-432.doi: 10.7525/j.issn.1673-5102.2020.03.014

• 研究报告 • 上一篇    

水稻盐碱逆境响应锌指蛋白基因OsZFP6表达特性及功能研究

刘佳丽1, 何明良2,3, 刘晨曦1, 廖栩1, 李秀峰2,3, 管清杰1   

  1. 1. 东北盐碱植被恢复与重建教育部重点实验室, 东北林业大学生命科学学院, 哈尔滨 150040;
    2. 中国科学院东北地理与农业生态研究所, 哈尔滨 150081;
    3. 中国科学院大学, 北京 100049
  • 收稿日期:2019-10-01 发布日期:2020-05-29
  • 通讯作者: 管清杰,E-mail:guanqingjie@nefu.edu.cn E-mail:guanqingjie@nefu.edu.cn
  • 作者简介:刘佳丽(1994-),女,硕士,主要从事生物化学与分子生物学方面的研究。
  • 基金资助:
    黑龙江省自然科学基金(C2017009);国家重点研究开发计划(2016YFC0501203);中央大学基础研究基金(2572016EAJ6);黑龙江省博士后科研发展基金(LBH-Q15004)

Zinc Finger Protein OsZFP6 Expression Features and Functions in Saline-alkali Stress Response

LIU Jia-Li1, HE Ming-Liang2,3, LIU Chen-Xi1, LIAO Xu1, LI Xiu-Feng2,3, GUAN Qing-Jie1   

  1. 1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040;
    2. Northeast Institute of Geography and Agricultural Ecology of the Chinese Academy of Sciences, Harbin 150081;
    3. University of the Chinese Academy of Sciences, Beijing 100049
  • Received:2019-10-01 Published:2020-05-29
  • Supported by:
    Natural Science Foundation of Heilongjiang Province of China(C2017009);National Key Research and Development Program of China(2016YFC0501203);Fundamental Research Funds for the Central Universities(2572016EAJ6);Heilongjiang Postdoctoral Scientific Research Developmental Fund(LBH-Q15004)

摘要: 筛选到C2HC型锌指蛋白,其编码304个氨基酸,命名为OsZFP6。分别使用碱性盐(NaHCO3)和H2O2对水稻两周龄幼苗进行胁迫处理,检测到OsZFP6相对表达量受其诱导,并在叶和根中出现差异,因此,推测OsZFP6参与相应的逆境响应调节途径。我们将一个绿色荧光蛋白(GFP)与之融合,发现OsZFP6定位在细胞核。此外,对过表达OsZFP6的水稻转基因植株进行NaHCO3胁迫处理,结果表明过表达植株均具有较强的耐受性;并且在株高、鲜重生理指标上,野生型植株低于过表达OsZFP6植株,在野生型植株体内的MDA、H2O2含量高于过表达植株;说明OsZFP6在响应NaHCO3胁迫时可以通过清除过氧化物来增强植物的抗性。初步推测OsZFP6基因参与响应碱性盐胁迫途径的调控,可能在转录调控中直接或间接发挥重要作用。

关键词: 锌指蛋白, 非生物胁迫, 水稻, 碱性盐

Abstract: C2HC type zinc finger protein was screened, encoded 304 amino acids and named OsZFP6. The expression level of OsZFP6 was increased by abiotic stress, including alkali(NaHCO3) and H2O2 treatment in two-week old rice leaves but decreased in roots. Thus, it was speculated that OsZFP6 was involved in the regulation corresponding reaction pathways of resistance. We fused a green fluorescent protein(GFP) with it and found that OsZFP6 was located in the nucleus. Moreover, rice overexpressed OsZFP6 were more tolerant to NaHCO3 treatments. The overexpressed plants had strong tolerance, and were higher than WT in plant height and fresh weight, but the contents of MDA and H2O2 in vivo were lower than non-transgenic plants, which indicated that OsZFP6 could enhance the resistance of plants by scavenging peroxide in response to NaHCO3 stress. Preliminarily, it was speculated that OsZFP6 gene participated in the regulation of resistance and it may play a vital role in transcription regulation.

Key words: zinc finger protein, abiotic stress, rice, basic salt

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