外源茉莉酸甲酯对干旱胁迫下狭叶黄芩光合和生理特性的影响

doi:10.7525/j.issn.1673-5102.2020.03.006

摘要/Abstract

摘要： 以狭叶黄芩为试验材料，测定了不同浓度（0.1、0.5、1、2 mmol·L^-1）茉莉酸甲酯对干旱胁迫下（15%，PEG-6000）狭叶黄芩表型特征、光合以及生理特性的影响。结果外源施加茉莉酸甲酯增强了狭叶黄芩的净光合速率、气孔导度和蒸腾速率。有效降低了狭叶黄芩受伤害等级。可以有效缓解降低了胞间CO₂浓度，其中施用2 mmol·L^-1茉莉酸甲酯9 d后对狭叶黄芩的净光合速率、蒸腾速率、气孔导度、胞间CO₂浓度的影响最为显著。处理组的游离脯氨酸含量低于CK。可溶性蛋白含量在前6 d的处理中低于CK，而在6 d以后的处理中显著高于CK。外源施加茉莉酸甲酯能够提高SOD、POD、CAT活性。对狭叶黄芩的光合参数和生理指标的研究结果表明，经外源施加的茉莉酸甲酯处理能够有效减缓干旱胁迫对狭叶黄芩的不良影响。

关键词: 狭叶黄芩, PEG-6000, 茉莉酸甲酯, 光合特性, 生理指标

Abstract: The effects of exogenous application of different concentrations(0.1, 0.5, 1 and 2 mmol·L^-1) of methyl jasmonate on phenotypic characteristics and photosynthesis and physiological characteristics under 15% PEG-6000 stress were studied with the Scutellaria regeliana as experimental materials. The results showed that:exogenous application of methyl jasmonate enhanced the net photosynthetic rate, stomatal conductance and transpiration rate of S.regeliana. It mainly plays a role in promoting these and effectively reduces the damage level of the S.regeliana. The intercellular CO₂ concentration was decreased, and the effect of 2 mmol·L^-1 methyl jasmonate on the net photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO₂ concentration of S.regeliana was the most significant. The free proline content of the treatment group lower than CK. The soluble protein content lower than CK in the first 6 d treatment, and was significantly higher than that of CK after 6 d treatment. Exogenous application of methyl jasmonate can increase the activity of SOD, POD and CAT as a whole. The results of photosynthetic parameters and physiological indexes of S.regeliana showed that exogenous application of methyl jasmonate could effectively alleviate the adverse effects of drought stress on S.regeliana.

Key words: Scutellaria regeliana, PEG-6000, methyl jasmonate, photosynthetic characteristics, physiological index

中图分类号:

S567.239

吴建慧, 范卫芳, 牛喆, 张静, 高鹏飞, 李文. 外源茉莉酸甲酯对干旱胁迫下狭叶黄芩光合和生理特性的影响[J]. 植物研究, 2020, 40(3): 360-367.

WU Jian-Hui, FAN Wei-Fang, NIU Zhe, ZHANG Jing, GAO Peng-Fei, LI Wen. Effects of Exogenous Methyl Jasmonate on Photosynthesis and Physiological Characteristics of Scutellaria regeliana under Drought Stress[J]. Bulletin of Botanical Research, 2020, 40(3): 360-367.

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