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植物研究 ›› 2020, Vol. 40 ›› Issue (2): 301-307.doi: 10.7525/j.issn.1673-5102.2020.02.018

• 研究报告 • 上一篇    下一篇

悬钩子属DNA条形码通用序列的初步筛选

巫伟峰1,2, 沈玺龙1, 陈哲1, 杨鼎元1, 李永霞1, 王瑶1, 张群英1   

  1. 1. 贵州省植物园, 贵阳 550000;
    2. 中国科学院庐山植物园, 九江 332900
  • 收稿日期:2019-08-24 出版日期:2020-03-15 发布日期:2020-03-06
  • 通讯作者: 张群英,E-mail:61138857@qq.com E-mail:61138857@qq.com
  • 作者简介:巫伟峰(1990-),男,研究实习员,主要从事悬钩子属植物研究。
  • 基金资助:
    贵州省科技厅黔科合成果项目([2019]4330号)

Preliminary Selection of Potential DNA Barcodes for Rubus

WU Wei-Feng1,2, SHEN Xi-Long1, CHEN Zhe1, YANG Ding-Yuan1, LI Yong-Xia1, WANG Yao1, ZHANG Qun-Ying1   

  1. 1. Guizhou Botanical Garden, Guiyang 550000;
    2. Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang 332900
  • Received:2019-08-24 Online:2020-03-15 Published:2020-03-06
  • Supported by:
    Science Technology Department Qinhe Achievement Project of Guizhou province(No.4330[2019])

摘要: 为了建立悬钩子属(Rubus)植物的DNA条形码分子鉴定技术,筛选获得适用于悬钩子属植物的通用条形码序列。该研究基于GenBank数据对ITS、ITS2、matK、rbcL、trnH-psbA、trnL-trnF 6个DNA条形码序列进行了遗传变异、barcoding gap、建树等评估分析。结果显示,trnH-psbA、matK、rbcL、rtnL-trnF的种内变异与种间变异差异较大,变异分辨率分别为97.32%、83.33%、79.07%、64.95%,存在较大的barcoding gap;NJ一致树分析显示,matK的单系性比例最高(67%),其次为trnH-psbA(64%),rtnL-trnF(43%),rbcL(30%)。结果表明,悬钩子属植物的matKtrnH-psbA序列种内变异与种间变异差异较大,能较好地区分不同物种,具有较大的鉴定潜力。建议将matKtrnH-psbA作为悬钩子属植物鉴定的核心条形码序列,rtnL-trnF、rbcL作为辅助条形码序列。

关键词: 悬钩子属, DNA条形码筛选, 物种鉴定, matK, trnH-psbA

Abstract: In order to establish a molecular identification technique of DNA barcoding for Rubus, and screening universal barcode sequences suitable for Rubus. With GenBank data we analyzed the genetic variation, barcoding gap and NJ consensus tree of six DNA barcoding sequences that contain ITS, ITS2, matK, rbcL, trnH-psbA and trnL-trnF. The intraspecific variation and interspecies variation of trnH-psbA, matK, rbcL and rtnL-trnF were different, and the variation resolutions were 97.32%, 83.33%, 79.07% and 64.95%, respectively. And trnH-psbA, matK, rbcL and rtnL-trnF had obvious barcoding gap; NJ consistent tree showed that matK had the highest proportion of solopathogenicity(67%), and then trnH-psbA(64%), rtnL-trnF(43%), rbcL(30%). The intraspecific variation and interspecies of matK and trnH-psbA sequences for Rubus are different, which can distinguish different species well with great identification potential. It is recommended to use matK and trnH-psbA as the core barcode sequences identified for Rubus, and rtnL-trnF and rbcL are used as auxiliary barcode sequences.

Key words: Rubus, DNA barcodes selection, species identification, matK, trnH-psbA

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