真菌联合稀碱预处理杜仲叶提取杜仲胶的工艺研究

doi:10.7525/j.issn.1673-5102.2019.05.018

植物研究 ›› 2019, Vol. 39 ›› Issue (5): 788-794.doi: 10.7525/j.issn.1673-5102.2019.05.018

真菌联合稀碱预处理杜仲叶提取杜仲胶的工艺研究

刘佩岩, 王思莹, 郭紫婧, 赵修华

东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040

收稿日期:2019-04-29 出版日期:2019-09-05 发布日期:2019-07-16
通讯作者: 赵修华 E-mail:xiuhuazhao@nefu.edu.cn
作者简介:刘佩岩(1993-),男,硕士研究生,主要研究方向为天然产物化学。
基金资助:
林业公益性行业科技专项（201504701-2-3）

Extraction Technology of Gutta-percha from Eucommia ulmoides Leaves Pretreated with Fungus and Dilute Alkali

LIU Pei-Yan, WANG Si-Ying, GUO Zi-Jing, ZHAO Xiu-Hua

Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin 150040

Received:2019-04-29 Online:2019-09-05 Published:2019-07-16
Supported by:
Forestry public welfare industry science and technology special(201504701-2-3)

摘要/Abstract

摘要： 以杜仲干叶为原料，经稀碱和绿色木霉联合处理以除去杜仲表面覆盖的角质层，同时洗脱一些可溶性杂质，并且破坏杜仲叶的细胞壁与纤维结构，再以石油醚作为提取溶剂，得到粗胶再经丙酮浸洗获得杜仲精胶。用0.45% NaOH在75℃下除去杜仲叶表面的角质层，并且同时脱除一些可溶性活性成分（如：多糖、黄酮类、京尼平苷等），之后得杜仲叶干燥，利用绿色木霉去破坏杜仲叶的细胞壁与纤维结构，在85℃条件下以石油醚作为提取溶剂提取杜仲胶。最后，经测得未处理前杜仲叶中的杜仲胶的含量为1.78%，0.45% NaOH稀碱预处理后含量为3.16%，绿色木霉发酵后含量为4.36%。预处理前，有机溶剂一次提取率为0.73%，纯度为83.7%。稀碱与真菌联合预处理后一次提取率为2.85%，纯度为96.6%。本工艺以稀碱与绿色木霉共同作用，减少了有机溶剂的用量，与传统工艺相比，更加的绿色环保，安全有效，为杜仲胶的提取提供了科学有效的依据。

关键词: 杜仲叶, 杜仲胶, 稀碱, 绿色木霉, 石油醚, 工艺优化

Abstract: The experiment was conducted to study the extraction and purification of high purity gutta-percha from Eucommia ulmoides leaves. The dried leaves of E.ulmoides were treated with dilute alkali and trichoderma viridis to remove the cuticle covering the surface of E.ulmoides, and some soluble impurities were eluted and the cell wall and fiber structure of the leaves were destroyed. The 0.45% NaOH under 75℃ was used to remove the surface of the barks of E.ulmoides corneous layer, as well as some soluble active ingredients(such as polysaccharides, flavonoids, Beijing's flat glycosides, etc.), after drying barks of E.ulmoides, and used green trichoderma viride to destroy the cell walls of the barks of E.ulmoides and fiber structure, and under 85℃ E.ulmoides was extracted by petroleum ether as solvent extraction of gutta-percha. Finally, the content of gutta-percha in the leaves of E.ulmoides before treatment was determined to be 1.78%, the content of dilute alkali of 0.45% NaOH after pretreatment was 3.16%, and the content of green trichoderma after fermentation was 4.36%. Before pretreatment, the yield of primary extraction and purity of organic solvent were 0.73% and 83.7%, respectively. After combined pretreatment, the yield of primary extraction was 2.85% and the purity was 96.6%. This process combined with dilute alkali and green trichophylla reduced the amount of organic solvent, and compared with the traditional process, it was more green, safe and effective.

Key words: Eucommia ulmoides, Eucommia ulmoides gum, dilute alkali, green trichoderma, petroleum ether, process optimization

中图分类号:

Q949.751.5

刘佩岩, 王思莹, 郭紫婧, 赵修华. 真菌联合稀碱预处理杜仲叶提取杜仲胶的工艺研究[J]. 植物研究, 2019, 39(5): 788-794.

LIU Pei-Yan, WANG Si-Ying, GUO Zi-Jing, ZHAO Xiu-Hua. Extraction Technology of Gutta-percha from Eucommia ulmoides Leaves Pretreated with Fungus and Dilute Alkali[J]. Bulletin of Botanical Research, 2019, 39(5): 788-794.

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真菌联合稀碱预处理杜仲叶提取杜仲胶的工艺研究

Extraction Technology of Gutta-percha from Eucommia ulmoides Leaves Pretreated with Fungus and Dilute Alkali

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