转录因子WRKY6和PR1在拟南芥胁迫记忆中的表达模式

doi:10.7525/j.issn.1673-5102.2019.05.014

摘要/Abstract

摘要： 植物暴露在细菌或其它微生物病原体下，会形成全身防御，称为系统获得性抗性SAR（Systemic Acquired Resistance），该系统可以在病原体二次侵染时有效抑制病原体对植物的伤害。其中，WRKY转录因子和病程相关蛋白PRs（Pathogenesis-related proteins）在植物抗病信号调控途径中起着重要作用。本研究以模式植物拟南芥为实验材料，对WRKY6和PR1（PATHOGENESIS RELATED）两个转录因子进行初步研究。首先，从拟南芥eFP数据库中获得WRKY6和PR1的基因表达数据，进行生物信息学分析，获得WRKY6和PR1基因在不同胁迫条件下的表达热图。其次，通过实时荧光定量PCR技术，比较了经过生物胁迫和非生物胁迫处理后WRKY6和PR1的基因表达水平。结果表明，拟南芥经过生物胁迫丁香假单胞菌[Pseudomonas syringae pv.tomato（P_st） DC3000]处理后，WRKY6和PR1的基因表达模式具有一定的相似性，然而经过非生物胁迫和机械损伤组合处理后，WRKY6和PR1基因又呈现出不同的表达模式。本研究初步探索了WRKY6和PR1基因的表达模式及其关系，为今后进一步研究系统性获得抗性应答机制提供了思路。

关键词: 拟南芥, WRKY6, PR1, 胁迫响应, 表达分析

Abstract: Plants exposed to bacteria or other microbic pathogens can develop a whole body's defense, also known as systemic acquired resistance(SAR), which can inhibit the growth of pathogens during a secondary infection. Transcription factors WRKY and PATHOGENESIS-RELATED proteins play an important role in plant disease resistance regulation pathway. WRKY6 and PATHOGENESIS RELATED1(PR1) are well-known stress response genes in Arabidopsis thaliana both encoding transcription factors. In this study, we obtained the gene expression data of WRKY6 and PR1 from A.thaliana eFP database, followed by bioinformatics analysis and generated the expression heat map of WRKY6 and PR1 under the abiotic stress and biotic stress. We compared the expression levels of WRKY6 and PR1 after various stress treatments. The results showed that, after Pseudomonas syringae pv.tomato(P_st) DC3000 treatment WRKY6 and PR1 had similar expression patterns, but after the combination of BTH and mechanical stress WRKY6 and PR1 had different expression pattern. Our results indicate that WRKY6 and PR1 react differently after diverse treatments, indicating that both genes act synergistic and independent of each other. It provides a way to further study the mechanism of systemic acquired resistance response in the future.

Key words: Arabidopsis thaliana, WRKY6, PR1, stress response, expression analysis

中图分类号:

S722.3+7

李爽, 熊樱, RALF M;ller-Xing, 邢倩. 转录因子WRKY6和PR1在拟南芥胁迫记忆中的表达模式[J]. 植物研究, 2019, 39(5): 752-759.

LI Shuang, XIONG Ying, RALF M;ller-Xing, XING Qian. Distinct Expression Patterns of WRKY6 and PR1 in Arabidopsis Stress Memory Assays[J]. Bulletin of Botanical Research, 2019, 39(5): 752-759.

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