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植物研究 ›› 2019, Vol. 39 ›› Issue (5): 752-759.doi: 10.7525/j.issn.1673-5102.2019.05.014

• 研究报告 • 上一篇    下一篇

转录因子WRKY6和PR1在拟南芥胁迫记忆中的表达模式

李爽, 熊樱, RALF Müller-Xing, 邢倩   

  1. 东北林业大学生命科学学院, 哈尔滨 150040
  • 收稿日期:2019-05-14 出版日期:2019-09-05 发布日期:2019-07-16
  • 通讯作者: 邢倩 E-mail:xingqian123@hotmail.com
  • 作者简介:李爽(1993-),女,硕士研究生,主要研究生物与非生物胁迫条件下的表观遗传记忆。
  • 基金资助:
    中央高校基本科研业务费专项资金资助(2572016DA03);黑龙江省科学基金项目(C216007)

Distinct Expression Patterns of WRKY6 and PR1 in Arabidopsis Stress Memory Assays

LI Shuang, XIONG Ying, RALF Müller-Xing, XING Qian   

  1. College of Life Science, Northeast Forestry University, Harbin 150040
  • Received:2019-05-14 Online:2019-09-05 Published:2019-07-16
  • Supported by:
    The Fundamental Research Funds for the Central Universities(2572016DA03);Natural Science Foundation of Heilongjiang Province(C216007)

摘要: 植物暴露在细菌或其它微生物病原体下,会形成全身防御,称为系统获得性抗性SAR(Systemic Acquired Resistance),该系统可以在病原体二次侵染时有效抑制病原体对植物的伤害。其中,WRKY转录因子和病程相关蛋白PRs(Pathogenesis-related proteins)在植物抗病信号调控途径中起着重要作用。本研究以模式植物拟南芥为实验材料,对WRKY6和PR1(PATHOGENESIS RELATED)两个转录因子进行初步研究。首先,从拟南芥eFP数据库中获得WRKY6和PR1的基因表达数据,进行生物信息学分析,获得WRKY6和PR1基因在不同胁迫条件下的表达热图。其次,通过实时荧光定量PCR技术,比较了经过生物胁迫和非生物胁迫处理后WRKY6和PR1的基因表达水平。结果表明,拟南芥经过生物胁迫丁香假单胞菌[Pseudomonas syringae pv.tomatoPst) DC3000]处理后,WRKY6和PR1的基因表达模式具有一定的相似性,然而经过非生物胁迫和机械损伤组合处理后,WRKY6和PR1基因又呈现出不同的表达模式。本研究初步探索了WRKY6和PR1基因的表达模式及其关系,为今后进一步研究系统性获得抗性应答机制提供了思路。

关键词: 拟南芥, WRKY6, PR1, 胁迫响应, 表达分析

Abstract: Plants exposed to bacteria or other microbic pathogens can develop a whole body's defense, also known as systemic acquired resistance(SAR), which can inhibit the growth of pathogens during a secondary infection. Transcription factors WRKY and PATHOGENESIS-RELATED proteins play an important role in plant disease resistance regulation pathway. WRKY6 and PATHOGENESIS RELATED1(PR1) are well-known stress response genes in Arabidopsis thaliana both encoding transcription factors. In this study, we obtained the gene expression data of WRKY6 and PR1 from A.thaliana eFP database, followed by bioinformatics analysis and generated the expression heat map of WRKY6 and PR1 under the abiotic stress and biotic stress. We compared the expression levels of WRKY6 and PR1 after various stress treatments. The results showed that, after Pseudomonas syringae pv.tomato(Pst) DC3000 treatment WRKY6 and PR1 had similar expression patterns, but after the combination of BTH and mechanical stress WRKY6 and PR1 had different expression pattern. Our results indicate that WRKY6 and PR1 react differently after diverse treatments, indicating that both genes act synergistic and independent of each other. It provides a way to further study the mechanism of systemic acquired resistance response in the future.

Key words: Arabidopsis thaliana, WRKY6, PR1, stress response, expression analysis

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