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植物研究 ›› 2019, Vol. 39 ›› Issue (2): 246-251.doi: 10.7525/j.issn.1673-5102.2019.02.010

• 研究报告 • 上一篇    下一篇

化学诱变剂EMS对知母种子萌发的影响

杨楠, 聂江力, 辛微, 夏徐, 郎玉洁, 裴毅   

  1. 天津农学院园艺园林学院, 天津 300384
  • 收稿日期:2018-10-25 出版日期:2019-03-05 发布日期:2019-03-20
  • 通讯作者: 聂江力 E-mail:njlnie@126.com
  • 作者简介:杨楠(1996-),女,硕士研究生,主要从事植物资源开发与利用。
  • 基金资助:
    天津市科技计划资助项目(17JCYBJC29800)

Effects of EMS on Seed Germination of Anemarrhena asphodeloides in the Simulated Environment

YANG Nan, NIE Jiang-Li, XIN Wei, XIA Xu, LANG Yu-Jie, PEI Yi   

  1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384
  • Received:2018-10-25 Online:2019-03-05 Published:2019-03-20
  • Supported by:
    Science and Technology Program Funding Project of Tianjin(17JCYBJC29800)

摘要: 以知母(Anemarrhena asphodeloides)种子为材料,分别采用1%、2%、4%、6%浓度的EMS处理6、8、12和24 h,在恒温培养箱内进行种子萌发实验,研究不同浓度的化学诱变剂甲基磺酸乙酯(ethyl methane sulfonate,EMS)处理对知母种子萌发的影响,筛选适宜的诱变浓度和诱变时间。结果表明:随EMS浓度的增加和处理时间的延长,知母种子发芽率呈现逐渐降低的趋势,以相对发芽率达到半致死浓度为标准,6%EMS浸种12和24 h可作为EMS诱导知母建立突变体库的适宜条件。

关键词: 化学诱变, 甲基磺酸乙酯(EMS), 知母, 种子萌发

Abstract: The seeds of Anemarrhena asphodeloides were treated with different contents and treatment times of EMS at 1%, 2%, 4%, 6% and 6, 8, 12, 24 h, respectively, in the constant temperature incubator in this experiment.We studied the effects of the different concentrations and treatment time of EMS on the seed germination characteristics of A.asphodeloides. The results of seed germination showed that the seed germination of A.asphodeloides reduced with theincreasings of the contents and the treatment time of EMS. With the relative germination rate reaching the semi-lethal concentration as the standard, the suitable condition for EMS treatment of A.asphodeloides seeds are the concentration of 6% and soaking seeds for 12 and 24 hours.

Key words: chemical mutagenesis, EMS, Anemarrhena asphodeloides, seed germination

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