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植物研究 ›› 2019, Vol. 39 ›› Issue (1): 69-77.doi: 10.7525/j.issn.1673-5102.2019.01.009

• 研究报告 • 上一篇    下一篇

不同喀斯特小生境中小蓬竹的遗传多样性

刘济明, 管睿婷, 王敏, 陈敬忠, 童炳丽, 武梦瑶   

  1. 贵州大学林学院, 贵阳 550025
  • 收稿日期:2018-09-19 出版日期:2019-01-15 发布日期:2019-01-31
  • 作者简介:刘济明(1963-),男,教授,博士生导师,主要从事野生动植物保护与利用和植物生态学研究。
  • 基金资助:
    贵州省国际科技合作计划项目"小蓬竹多样性及其根际生态研究"[黔科合外G字(2013)7010];黔南州社会发展科技计划项目"黔南石漠化生态治理与示范研究

Genetic Diversity of Ampelocalamus luodianensis in Different Microhabitats

LIU Ji-Ming, GUAN Rui-Ting, WANG Min, CHEN Jing-Zhong, TONG Bing-Li, WU Meng-Yao   

  1. College of Forestry, Guizhou University, Guiyang 550025
  • Received:2018-09-19 Online:2019-01-15 Published:2019-01-31
  • Supported by:
    International Science and Technology Cooperation Project of Guizhou Province[(2013)7010];Science and Technology for Social Development Project of Qiannan State

摘要: 通过RAPD标记法对5类喀斯特小生境中小蓬竹的遗传结构及其多样性进行了分析。RAPD随机引物8个扩增共得87个清晰度高且重复性好的条带,分子量200~3 000 bp,含多态68条,物种PPL=78.16%,Nei's基因多度H=0.291 3,Shannon多样性指数I=0.431 0。土面(TM)生长的小蓬竹遗传多样性水平最高(PPL=47.13%,H=0.195 3,I=0.283 4),石缝(SF)中的小蓬竹遗传多样性水平最低(PPL=39.08%,H=0.147 4,I=0.219 2)。POPGENE 32分析5类小生境中小蓬竹的遗传分化系数Gst=0.415 1,基因流Nm=0.704 5。5类小生境的小蓬竹间遗传相似系数(I)变化范围0.777 3~0.999 4,平均为0.865 6;遗传距离(D)变化范围0.000 6~0.251 9,平均为0.148 3。各类小生境的遗传多样性水平PPL均值为43.91%,5类小生境间的遗传分化Gst=0.405 4。研究结果说明,小生境间的差异增加了小蓬竹的遗传多样性。

关键词: 小蓬竹, RAPD-PCR, 遗传多样性, 小生境, 濒危机制, 保护策略

Abstract: By random amplified polymorphic DNA(RAPD) markers analysis, 5 kinds of karst microhabitats of Ampelocalamus luodianensis was conducted to study genetic diversity and genetic structure. Through 8 RAPD primers, 87 discernible DNA fragments in all weighted from 200-3 000 bp were cloned, and 68 in those were polymorphic loci, with percentage of polymorphic loci at the species level, PPL=78.16%, Nei's index H=0.291 3, and Shannon's Information Index I=0.431 0. The A.luodianensis grew on soil surface(TM) possessed the highest level of genetic diversity(PPL=47.13%, H=0.195 3 and I=0.283 4), while the A.luodianensis in stone crevice exhibited the lowest levels of genetic diversity(PPL=39.08%, H=0.147 4 and I=0.219 2). Coefficient of genetic differentiation (Gst) was 0.415 1(from the POPGENE32), and the gene flows(Nm) was 0.704 5. Among the 5 microhabitats, genetic identity varied from 0.777 3-0.999 4, genetic distance was different from 0.000 6-0.251 9, and the mean of them were 0.865 6-0.148 3 severally. Genetic diversity in niches at mean value was with PPL of 43.91%. Coefficient of genetic variation in niches was with Gst of 0.4054. The results indicated that differences of microhabitats increased the genetic diversity of A.luodianensis.

Key words: Ampelocalamus luodianensis, RAPD-PCR, genetic diversity, microhabitat, endangered mechanism, conservation strategies

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