白桦APETALA2基因启动子的克隆及其表达分析

doi:10.7525/j.issn.1673-5102.2018.04.007

摘要/Abstract

摘要： APETALA2（AP2）转录因子亚家族普遍存在于植物中，参与植株的生长发育、胁迫应答和多种生理生化反应的信号传导。本研究从白桦（Betula platyphylla Suk.）基因组中克隆了AP2基因2 308 bp的启动子序列，生物信息学分析发现，该序列除具有TATA-box和CAAT box等高等植物普遍具有的保守元件外，还具有大量光响应元件和激素响应元件，如响应赤霉素、脱落酸、茉莉酸甲酯等的元件。将白桦AP2基因启动子克隆至pBI121-35S：：GUS植物表达载体中，命名为pBI121-proAP2：：GUS，用农杆菌介导法侵染白桦和拟南芥，并进行GUS染色分析，结果表明AP2基因启动子驱动下的GUS报告基因在整个拟南芥中都表达，在白桦的营养器官和雌花种翅及花柄中也有表达，说明其具有启动活性，可能参与该器官的发育。

关键词: 白桦, AP2, 启动子, GUS组织化学染色

Abstract: The APETALA2(AP2) transcription factor subfamily is ubiquitous in plants, and involved in the plant growth and development, stress response and signal transduction of various physiological and biochemical reactions. The full-length promoter sequence of AP2 gene was cloned from the genome of Betula platyphylla Suk. By bioinformatics analysis, this sequence has a large number of conserved components such as TATA-box and CAAT box, but also light responsive elements and hormone responsive elements, such as elements that respond to gibberellins, abscisic acid, and methyl jasmonate. The promoter of AP2 gene of B.platyphylla was cloned into pBI121-35S::GUS plant expression vector, named pBI121-proAP2::GUS, Agrobacterium-mediated infection of B.platyphylla and A.thaliana, and by GUS staining analysis, the GUS reporter gene driven by the promoter of AP2 gene is expressed throughout A.thaliana and is expressed in vegetative organs and female flower wings and in flower stalk of B.platyphylla, indicating that it has a priming activity that may participate in the development of this organ.

Key words: Betula platyphylla, AP2, promoter, GUS histochemical staining

中图分类号:

Q943.2

赵桐, 胡晓晴, 朱德财, 田晶, 辛琪琪, 刘雪梅. 白桦APETALA2基因启动子的克隆及其表达分析[J]. 植物研究, 2018, 38(4): 535-542.

ZHAO Tong, HU Xiao-Qing, ZHU De-Cai, TIAN Jing, XIN Qi-Qi, LIU Xue-Mei. Cloning and Expression Analysis of APETALA2 Gene Promoter from Betula platyphylla[J]. Bulletin of Botanical Research, 2018, 38(4): 535-542.

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