外源GA3对毛竹实生苗茎秆生长及CesA基因表达的影响

doi:10.7525/j.issn.1673-5102.2017.05.015

植物研究 ›› 2017, Vol. 37 ›› Issue (5): 744-750.doi: 10.7525/j.issn.1673-5102.2017.05.015

外源GA₃对毛竹实生苗茎秆生长及CesA基因表达的影响

姜勇^1,2, 胡尚连^1,2, 曹颖^1,2, 卢学琴^1,2, 黄艳^1,2, 徐刚^1,2

1. 西南科技大学生命科学与工程学院植物细胞工程实验室, 绵阳 621010;
2. 四川省生物质资源利用与改性工程技术研究中心, 绵阳 621010

收稿日期:2017-02-21 出版日期:2017-09-15 发布日期:2017-09-15
通讯作者: 胡尚连,E-mail:hushanglian@126.com E-mail:hushanglian@126.com
作者简介:姜勇(1991-),男,硕士研究生,从事植物遗传与品种改良研究。
基金资助:
国家自然科学基金（31400257，31400333）；四川省"十三五"重点公关项目（2016NYZ0038）；四川省生物质资源利用与改性工程技术研究中心基金（12zxsk07，13zxsk01）；西南科技大学研究生创新基金（16ycx068）

Effects of Exogenous GA₃ on the Growth of Stem and CesA Gene Expression in Phyllostachys edulis Seedling

JIANG Yong^1,2, HU Shang-Lian^1,2, CAO Ying^1,2, LU Xue-Qin^1,2, HUANG Yan^1,2, XU Gang^1,2

1. Lab of Plant Cell Engineering, School of life science and Engineering, Southwest University of Science and Technology, Mianyang 621010;
2. Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan Province, Mianyang 621010

Received:2017-02-21 Online:2017-09-15 Published:2017-09-15
Supported by:
National Natural Science Foundation of China(31400257,31400333);Breeding Program Fund project by the 13th Five-year plan of Sichuan province(2016NYZ0038);Fund of Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan province(12zxsk07,13zxsk01);Graduate innovation fund of Southwest University of Science and Technology(16ycx068)

摘要/Abstract

摘要： 以毛竹实生苗为试验材料，研究不同浓度外源GA₃（0，0.1，0.5，1 μmol·L^-1）对毛竹生长及CesA基因表达的影响。结果表明，与未经GA₃处理相比，施用外源GA₃后，毛竹实生苗茎节间和纤维细胞显著伸长，初生壁纤维素合成相关基因PeCesA2和PeCesA6相对表达量明显上调，次生壁相关基因PeCESA4和PeCESA4-1显著下调，傅里叶红外光谱分析（FTIR），发现毛竹茎秆纤维素特征峰强度（1 060，1 160及1 373 cm^-1）随着GA₃浓度升高而逐渐增强，表明施用外源GA₃能够影响毛竹茎秆CesA基因表达，PeCesA2和PeCesA6的表达与外源GA₃促进纤维细胞伸长的过程存在一定联系。

关键词: 毛竹实生苗, GA₃, 纤维细胞, CesA基因

Abstract: We studied the growth of stem and CesA gene expression from the seedling in Phyllostachys edulis under the different concentration of exogenous GA₃(0, 0.1, 0.5, 1 μmol·L^-1). Compared with without GA₃ treatment, the internode and fiber cells of stem from the seedling elongated significantly, after treated by exogenous GA₃. The relative expression of primary wall-related PeCesA2 and PeCesA6 genes were significantly up-regulated, while that of secondary wall-related PeCesA4 and PeCesA4-1 genes were significantly down-regulated. By Fourier transform infrared spectroscopy(FTIR), the absorption intensity of characteristic cellulose peak(1 060, 1 160 and 1 373 cm^-1) from the stems enhanced with the increase of GA₃ concentration. Signifying the expression of CesA gene could be affected by the exogenous GA₃. The expression of PeCesA2 and PeCesA6 genes could be related to the process of fiber cells elongation.

Key words: Phyllostachys edulis seedling, GA₃, fiber cell, CesA gene

中图分类号:

S795.9

姜勇, 胡尚连, 曹颖, 卢学琴, 黄艳, 徐刚. 外源GA₃对毛竹实生苗茎秆生长及CesA基因表达的影响[J]. 植物研究, 2017, 37(5): 744-750.

JIANG Yong, HU Shang-Lian, CAO Ying, LU Xue-Qin, HUANG Yan, XU Gang. Effects of Exogenous GA₃ on the Growth of Stem and CesA Gene Expression in Phyllostachys edulis Seedling[J]. Bulletin of Botanical Research, 2017, 37(5): 744-750.

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外源GA₃对毛竹实生苗茎秆生长及CesA基因表达的影响

Effects of Exogenous GA₃ on the Growth of Stem and CesA Gene Expression in Phyllostachys edulis Seedling

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