山桐子(Idesia polycarpa Maxim)果实多酚的抗炎、抗菌活性研究

doi:10.7525/j.issn.1673-5102.2016.06.021

摘要/Abstract

摘要： 多酚类物质是植物体中重要的次级代谢产物，为充分利用山桐子果实，将果实中多酚类物质提取出来，并对其抗炎、抗菌效果进行研究。利用LSP诱导RAW264.7细胞产生炎症，经不同浓度给药后对MTT、细胞产生NO量进行测定；选取粪肠球菌、枯草芽孢杆菌、鼠伤寒沙门氏菌进行抗菌实验，判断抑菌效果。结果表明：山桐子果实中总多酚含量为6.6 mg·g^-1；给药组浓度依次为50、100和200 μg·mL^-1，随着给药浓度的增加RAW264.7细胞活力值也在增大，NO释放量随着给药浓度的升高降低，山桐子果实多酚对选取的三种细菌抑菌能力由强到弱为：枯草芽孢杆菌 > 沙门氏菌 > 粪肠球菌。

关键词: 山桐子果实, 多酚, 抗炎, 抗菌

Abstract: Polyphenols are important secondary metabolites in plant. For full use of the fruit, polyphenols in Ldesia palycarpa Maxim fruit were extracted and the anti-inflammatory activity and antibacterial effect were studied. LSP-induced RAW264.7 inflammatory cells were treated with different concentration polyphenols and the anti-inflammatory effect was determined by MTT assays and NO amount of RAW264.7 cells. Enterococcus faecalis, Bacillus subtilis, Salmonella typhimurium were selected for antimicrobial experiment to analyze antibacterial activity. The content of polyphenols of L.palycarpa Maxim fruit was 6.6 mg·g^-1. Under the administration concentrations with 50, 100 and 200 μg·mL^-1, along with the increase of administration concentration, RAW264.7 cells viability value was increased and the release of NO from RAW264.7 cells was also decreased. The antibacterial ability of polyphenols in L.palycarpa fruit of selected three kinds of bacteria in the descending order of B.subtilis, S.typhimurium and E.faecalis.

Key words: Idesia polycarpa fruit, polyphenols, anti-inflammatory activity, antibacterial activity

中图分类号:

Q949.759.3

昝鹏, 张琳, 祖元刚, 杨磊, 丛赢. 山桐子(Idesia polycarpa Maxim)果实多酚的抗炎、抗菌活性研究[J]. 植物研究, 2016, 36(6): 955-960.

ZAN Peng, ZHANG Lin, ZU Yuan-Gang, YANG Lei, CONG Ying. Anti-inflammation and Antibacterial Activity of Polyphenols in Idesia polycarpa Maxim. Fruit[J]. Bulletin of Botanical Research, 2016, 36(6): 955-960.

参考文献

1. 祝志勇,王强,阮晓,等.不同地理居群山桐子的果实含油率与脂肪酸含量[J].林业科学,2010,46(5):176-180. Zhu Z Y,Wang Q,Ruan X,et al.Analysis of oil rate and fatty acids content of Idesia palycarpa fruits from different geographical populations[J].Scientia Silvae Sinicae,2010,46(5):176-180.
2. 吴志文,谢双喜,刘青,等.山桐子的研究进展及应用前景[J].贵州农业科学,2010,38(1):161-164. Wu Z W,Xie S X,Liu Q,et al.Research progress and application prospect of Idesia polycarpa[J].Guizhou Agricultural Sciences,2010,38(1):161-164.
3. 杨志玲,王开良,谭梓峰.值得开发的几种野生木本油料树种[J].林业科技开发,2003,17(2):41-43. Yang Z L,Wang K L,Tan Z F.Introduction on several wild valuable oil trees[J].China Forestry Science and Technology,2003,17(2):41-43.
4. Wang Q Y,Du K F,Jia C M,et al.Optimization of linoleic acid from Idesia polycarpa Maxim.var.vestita Diels by response surface methodology[J].Advanced Materials Research,2011,396-398:1222-1227.
5. Yang F X,Su Y Q,LI X H,et al.Preparation of biodiesel from Idesia polycarpa var.vestita fruit oil[J].Industrial Crops and Products,2009,29(2-3):622-628.
6. 昝志惠,高艳梅,孙墨珑.核桃楸单宁提取及其抗氧化性[J].植物研究,2015,35(3):431-435. Zan Z H,Gao Y M,Sun M L.Extraction and antioxidant activity of tannic in Juglans mandshurica Maxim[J].Bulletin of Botanical Research,2015,35(3):431-435.
7. Shi R,Zhang Q Y,Vriesekoop F,et al.Preparation of organogel with tea polyphenols complex for enhancing the antioxidation properties of edible oil[J].Journal of Agricultural and Food Chemistry,2014,62(33):8379-8384.
8. Kazeem M I,Akanji M A,Hafizur R M,et al.Antiglycation,antioxidant and toxicological potential of polyphenol extracts of alligator pepper,ginger and nutmeg from Nigeria[J].Asian Pacific Journal of Tropical Biomedicine,2012,2(9):727-732.
9. Bubonja-sonje M,Giacometti J,Abram M.Antioxidant and antilisterial activity of olive oil,cocoa and rosemary extract polyphenols[J].Food Chemistry,2011,127(4):1821-1827.
10. Sakagami H,Takeda K,Makino Y,et al.Partial purification of novel differentiation-inducing substance(s) from hot water extract of Japanese pine cone[J].Japanese Journal of Cancer Research:Gann,1986,77(1):59-64.
11. Korkina L G,Pastore S,Dellambra E,et al.New molecular and cellular targets for chemoprevention and treatment of skin tumors by plant polyphenols:a critical review[J].Current Medicinal Chemistry,2013,20(7):852-868.
12. Fuhrman B,Volkova N,Aviram M.Pomegranate juice polyphenols increase recombinant paraoxonase-1 binding to high-density lipoprotein:studies in vitro and in diabetic patients[J].Nutrition,2010,26(4):359-366.
13. Leiro J M,Varela M,Piazzon M C,et al.The anti-inflammatory activity of the polyphenol resveratrol may be partially related to inhibition of tumour necrosis factor-α (TNF-α) pre-mRNA splicing[J].Molecular Immunology,2010,47(5):1114-1120.
14. Al-muammar M N,Khan F.Obesity:the preventive role of the pomegranate(Punica granatum)[J].Nutrition,2012,28(6):595-604.
15. Lamothe S,Azimy N,Bazinet L,et al.Interaction of green tea polyphenols with dairy matrices in a simulated gastrointestinal environment[J].Food & Function,2014,5(10):2621-2631.
16. Kim S H,Jang Y P,Sung S H,et al.Inhibitory activity of phenolic glycosides from the fruits of Idesia polycarpa on lipopolysaccharide-induced nitric oxide production in BV2 microglia[J].Planta Medica,2007,73(2):167-169.
17. Jung M H,Yoo J M,Kang Y J,et al.Idesolide,an isolate of Idesia polycarpa,inhibits apoptosis through induction of intracellular heat shock protein 70 in C2C12 muscle cells[J].Biological & Pharmaceutical Bulletin,2010,33(6):1063-1066.
18. Chai S C,Hooshmand S,Saadat R L,et al.Daily apple versus dried plum:impact on cardiovascular disease risk factors in postmenopausal women[J].Journal of the Academy of Nutrition and Dietetics,2012,112(8):1158-1168.
19. 李占君,张琳,祖元刚,等.二氧化碳超临界提取塔拉籽油及其品质分析[J].植物研究,2015,35(3):467-470. Li Z J,Zhang L,Zu Y G,et al.Tara oil extraction by supercritical carbon dioxide and its quality[J].Bulletin of Botanical Research,2015,35(3):467-470.
20. Pérez-jiménez J,Saura-calixto F.Macromolecular antioxidants or non-extractable polyphenols in fruit and vegetables:intake in four European countries[J].Food Research International,2015,74:315-323.
21. 陈平波,李霞.低浓度NO对高表达转玉米C₄型pepc水稻光合的促进[J].植物研究,2012,32(4):402-409. Chen P B,Li X.Promotion of photosynthesis of transgenic rice plant with overexpressing C₄ pepc from maize under low concentration NO[J].Bulletin of Botanical Research,2012,32(4):402-409.
22. Kolb H,Kolb-bachofen V.Nitric oxide in autoimmune disease:cytotoxic or regulatory mediator?[J].Immunology Today,1998,19(12):556-561.
23. Sharma J N,Al-omran A,Parvathy S S.Role of nitric oxide in inflammatory diseases[J].Inflammopharmacology,2007,15(6):252-259.
24. Pfeilschifter J,Eberhardt W,Beck K F.Regulation of gene expression by nitric oxide[J].Pflügers Archiv,2001,442(4):479-486.
25. Kang N J,Shin S H,Lee H J,et al.Polyphenols as small molecular inhibitors of signaling cascades in carcinogenesis[J].Pharmacology & Therapeutics,2011,130(3):310-324.
26. Byun E H,Omura T,Yamada K,et al.Green tea polyphenol epigallocatechin-3-gallate inhibits TLR2 signaling induced by peptidoglycan through the polyphenol sensing molecule 67-kDa laminin receptor[J].FEBS Letters,2011,585(5):814-820.
27. Vaquero M J R,Aredes Fernández P A A,De Nadra M C M,et al.Phenolic compound combinations on E.coli viability in a meat system[J].Journal of Agricultural and Food Chemistry,2010,58(10):6048-6052.
28. Jayaraman P,Sakharkar M K,Lim C S,et al.Activity and interactions of antibiotic and phytochemical combinations against Pseudomonas aeruginosa in vitro[J].International Journal of Biological Sciences,2010,6(6):556-568.
29. Saavedra M J,Borges A,Dias C,et al.Antimicrobial activity of phenolics and glucosinolate hydrolysis products and their synergy with streptomycin against pathogenic bacteria[J].Medicinal Chemistry,2010,6(3):174-183.
30. Jeandet P,Douillet-breuil A C,Bessis R,et al.Phytoalexins from the Vitaceae:biosynthesis,phytoalexin gene expression in transgenic plants,antifungal activity,and metabolism[J].Journal of Agricultural and Food Chemistry,2002,50(10):2731-2741.
31. 李永义.茶多酚对氧化应激仔猪的保护作用及机制研究[D].雅安:四川农业大学,2011. Li Y Y.Protective effects of tea polyphenols for weaned pigs challenged with oxidative stress[D].Ya'an:Sichuan Agricultural University,2011.
32. Debprasad C,Hemanta M,Paromita B,et al.Inhibition of NO₂,PGE₂,TNF-α,and iNOS EXpression by Shorea robusta L.:an ethnomedicine used for anti-inflammatory and analgesic activity[J].Evidence-Based Complementary and Alternative Medicine,2012,2012:254849.

[1]	高媛, 邢文馨, 王慧梅. 大孔树脂纯化岩高兰多酚的工艺研究[J]. 植物研究, 2021, 41(4): 626-632.
[2]	张力凡, 薛煜, 王岩, 杨光鑫, 杨光, 邸雪颖. 樟子松乙醇提取物体外抗氧化活性研究[J]. 植物研究, 2019, 39(3): 458-465.
[3]	倪玉娇, 赵春建, 李春英, 李国春, 王楷婷, 张玉坤. 超声辅助低共熔溶剂提取沙棘籽粕多酚的工艺优化[J]. 植物研究, 2017, 37(3): 474-480.
[4]	丛赢, 张琳, 祖元刚, 杨磊, 昝鹏. 油樟(Cinnamomum longepaniculatum)精油的抗炎及抗氧化活性初步研究[J]. 植物研究, 2016, 36(6): 949-954.
[5]	祖元刚, 胡艳, 姜守刚. 红松多酚物质的提取工艺及其抗炎活性初步研究[J]. 植物研究, 2016, 36(4): 634-640.
[6]	高银祥1,2；祖元刚1；杨逢建1；于雪莹1；姚丽萍1*. 向日葵植株中次生代谢产物分布及积累规律研究[J]. 植物研究, 2014, 34(6): 782-786.
[7]	严俊鑫;关桦楠;迟德富*;张永强;张丹. 机械损伤对重瓣玫瑰防御酶的诱导[J]. 植物研究, 2013, 33(4): 499-503.
[8]	谢久祥;林恭华;都玉蓉;余雪峰;索有瑞;张同作*. 油松不同部位多酚与单宁的含量比较研究[J]. 植物研究, 2012, 32(2): 243-247.
[9]	王吉凤;李青;包欣. 芍药组织培养中污染现象的克服[J]. 植物研究, 2012, 32(1): 84-90.
[10]	张俞;刘婷婷;刘洋;杨磊;祖元刚*. 蒙古栎种壳多酚的超声辅助提取及抗氧化能力[J]. 植物研究, 2010, 30(5): 623-628.
[11]	李玉杰;刘晓蕾;刘霞;吴楠;彭霄;梁璐;付玉杰;*. 玫瑰精油的化学成分及其抗菌活性[J]. 植物研究, 2009, 29(4): 488-491.
[12]	范现丽;王高;王宏;范建芝;申晓辉*. 香桃木叶片粗多酚抗氧化活性的研究[J]. 植物研究, 2009, 29(3): 375-379.
[13]	王　微;吴　楠;付玉杰;祖元刚*. 薄荷精油抗菌活性研究[J]. 植物研究, 2007, 27(5): 626-629.
[14]	陈丽艳;施晓光;付玉杰*;候春莲;王　微;刘君星. 甘草根茎乙醇提取物抗菌活性研究[J]. 植物研究, 2006, 26(2): 229-232.
[15]	胡风庆, 王森林, 马辉, 王关林. 东北红豆杉细胞培养物PPO活性研究[J]. 植物研究, 2001, 21(4): 583-586.