5种木兰科植物的DNA-C值及倍性分析

doi:10.7525/j.issn.1673-5102.2016.06.008

摘要/Abstract

摘要： 以紫玉兰为内标或外标，利用流式细胞术测定了杂交马褂木（Liriodendron chinense×tulipifera）、深山含笑（Michelia maudiae）、长蕊含笑（Michelia longistamina）、广玉兰（Magnolia grandiflora）和二乔玉兰（Magnolia soulangeana）5种木兰科植物的DNA-C值及倍性。结果表明，两种含笑属植物及杂交马褂木均为二倍体，广玉兰为六倍体，二乔玉兰为四倍体，5种植物的C值各不相同，最大的是广玉兰，最小的是杂交马褂木，它们的C值大小属于小到中等水平。

关键词: 流式细胞术, DNA-C值, 倍性, 木兰科

Abstract: In this study, the C-value and ploidy of five species in Magnoliaceae was estimated by flow cytometry using Magnolia liliiflora as the reference. The results showed that the two species of Michelia are diploid, Magnolia grandiflora is hexaploid, and M.soulangeana is tetraploid. The C-Values of five species are different from each other. The C-value of M.grandiflora is the largest among the five species investigated, while the C-value of Liriodendron chinense×tulipifera is the smallest. The sizes of C-values of the five species are small to medium level.

Key words: flow cytometry, C-value, ploidy, Magnoliaceae

中图分类号:

Q949.747.1

赵青, 张强, 周鹏, 林峰, 方炎明. 5种木兰科植物的DNA-C值及倍性分析[J]. 植物研究, 2016, 36(6): 853-859.

ZHAO Qing, ZHANG Qiang, ZHOU Peng, LIN Feng, FANG Yan-Ming. Analysis of DNA C-value and Ploidy of Five Species in Magnoliaceae[J]. Bulletin of Botanical Research, 2016, 36(6): 853-859.

参考文献

1. 刘玉壶.木兰科植物及其珍稀濒危种类的迁地保护[J].热带亚热带植物学报,1997,5(2):1-12. Liu Y H.Ex situ conservation of Magnoliaceae including its rare and endangered species[J].Journal of Tropical and Subtropical Botany,1997,5(2):1-12.
2. 方炎明.植物学[M].北京:中国林业出版社,2005:284. Fang Y M.Botany[M].Beijing:China forestry publishing house,2005:284.
3. 王献溥,蒋高明.中国木兰科植物受威胁的状况及其保护措施[J].植物资源与环境学报,2001,10(4):43-47. Wang X P,Jiang G M.The threatened status and protected measures of Magnoliaceae species in China[J].Journal of Plant Resources and Environment,2001,10(4):43-47.
4. Bai C K,Alverson W S,Follansbee A,et al.New reports of nuclear DNA content for 407 vascular plant taxa from the United States[J].Annals of Botany,2012,110(8):1623-1629.
5. Lysák M A,Doleželova M,Horry J P,et al.Flow cytometric analysis of muclear DNA content in Musa[J].Thero Appl Genet,1999,98:1344-1350.
6. Arumuganathan K,Earle E D.Nuclear DNA content of some important plant species[J].Plant Molecular Biology Reporter,1991,9(3):208-218.
7. Greilhuber J,Doležel J,Lysák M A,et al.The origin,evolution and proposed stabilization of the terms‘genome size’and‘C-value’to describe nuclear DNA contents[J].Annals of Botany,2005,95:255-260.
8. 柳觐,孔广红,等.基于流式细胞术的澳洲坚果基因组C值测定[J].中国农学通报,2013,29(34):96-101. Liu J,Kong G H,Ni S B,et al.Estimation of genomic C value of Macadamia integrifolia spp.by flow cytometry[J].Chinese Agricultural Science Bulletin,2013,29(34):96-101.
9. Chen G Q,Guo S L,Yin L P.Applying DNA C-values to evaluate invasiveness of angiosperms:validity and limitation[J].Biological Invasions,2010,12(5):1335-1348.
10. Hui H,Yan T,Zhang Q J,et al.Genome size variation among and within Camellia species by using flow cytometric analysis[J].PLoS ONE,2013,8(5):1-12.
11. 施春晖,徐兰兰,王晓庆,等.流式细胞仪鉴定猕猴桃倍性技术研究[J].植物研究,2014,34(6):845-849. Shi C H,Xu L L,Wang X Q,et al.Identification of kiwifruit ploidy using flow cytometry[J].Bulletin of Botanical Research,2014,34(6):845-849.
12. Bennett M D,Leitch I J.Nuclear DNA amounts in angiosperms:targets,trends and tomorrow[J].Annals of Botany,2011,107(3):467-590.
13. Doležel J,Greilhuber J,Suda J.Estimation of nuclear DNA content in plants using flow cytometry[J].Nature Protocols,2007,2(9):2233-2244.
14. Galbraith D.Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values[J].Cytometry Part A,2009,75A:692-698.
15. 王亚玲,张寿洲,李勇,等.木兰科13个分类群和12个杂交组合的染色体数目[J].植物分类学报,2005,43(6):545-551. Wang Y L,Zhang S Z,Li Y,et al.Chromosome numbers of 13 taxa and 12 crossing combinations in Magnoliaceae[J].Acta Phytotaxonomica Sinica,2005,43(6):545-551.
16. 龚洵,张国莉,潘跃芝.木兰科两个杂交组合的细胞学研究[J].园艺学报,2003,30(5):615-617. Gong X,Zhang G L,Pan Y Z.A cytological study on two hybridized combinations of Magnoliaceae[J].Acta Horticulturae Sinica,2003,30(5):615-617.
17. 孟爱平,王恒昌,李建强,等.中国木兰科11属40种植物的核形态研究[J].植物分类学报,2006,44(1):47-63. Meng A P,Wang H C,Li J Q,et al.A karyomorphological study of 40 species in 11 genera of the Magnoliaceae from China[J].Acta Phytotaxonomica Sinica,2006,44(1):47-63.
18. 方其,印丽萍,郭水良.应用流式细胞术测定植物DNA C-值的实验方法研究[J].植物检疫,2011,25(2):40-44. Fang Q,Yin L P,Guo S.On method of application of flow cytometry to determine plant DNA C-value[J].Plant Quarantine,2011,25(2):40-44.
19. 田新民,周香艳,弓娜.流式细胞术在植物学研究中的应用-检测植物核DNA含量和倍性水平[J].中国农学通报,2011,27(9):21-27. Tian X M,Zhou X Y,Gong N.Applications of flow cytometry in plant research-analysis of nuclear DNA content and ploidy level in plant cells[J].Chinese Agricultural Science Bulletin,2011,27(9):21-27.
20. Yan J J,Zhang J B,Sun K Y,et al.Ploidy level and DNA content of Erianthus arundinaceus as determined by flow cytometry and the association with biological characteristics[J].PLoS ONE,2016,11(3):1-14.
21. 李彩琴,王泽槐,徐咏珊,等.流式细胞术细胞核分离缓冲液的改良及大、小果型荔枝幼果和果皮细胞分裂活性比较[J].园艺学报,2011,38(9):1781-1790. Li C Q,Wang Z H,Xu Y S,et al.Improvement of the cell nuclei suspensions for flow cytometry and comparison on the cell division activity of litchi fruitlet and pericarp which having different final fruit size[J].Acta Horticulturae Sinica,2011,38(9):1781-1790.
22. Ilia J,Mark W,Michael D.Phylogenetic analysis of DNA C-values provides evidence for a small ancestral genome size in flowering plants[J].Annals of Botany,1998,82(Supplement A):85-94.
23. Parris J K,Ranney T G,Knap H T,et al.Ploidy levels,relative genome sizes,and base pair composition in Magnolia[J].Journal of the American Society for Horticultural Science,2010,135(6):533-547.
24. 叶林江,张志荣,孙志霞,等.木兰科主要属种核DNA含量(2C值)的检测[J].植物分类与资源学报,2015,37(5):605-610. Ye J L,Zhang Z R,Sun Z X,et al.The determination of nuclear DNA content(2C-value) on some representative genus and species of Magnoliaceae[J].Plant Diversity and Resources,2015,37(5):605-610.
25. 吴丽萍,唐岩,李颖岳,等.枣和酸枣基因组大小测定[J].北京林业大学学报,2013,35(3):77-83. Wu L P,Tang Y,Li Y Y,et al.Estimation of genome size of Ziziphus jujuba and Z.acdiojujuba[J].Journal of Beijing Forestry University,2013,35(3):77-83.
26. 张新华,夏念和.木兰科植物染色体数目报道[J].热带亚热带植物学报,2005,13(6):516-518. Zhang X H,Xia N H.Chromosome numbers of five species and one hybrid in Magnoliaceae[J].Acta Phytotaxonomica Sinica,2005,13(6):516-518.
27. 沈慧,黄建,佟岩,等.枣基因组大小研究[J].西北林学院学报,2016,31(3):138-142. Shen H,Huang J,Tong Y,et al.Comparative analysis of genome sizes of Chinese jujube[J].Journal of Northwest Forestry University,2016,31(3):138-142.
28. Price H J,Morgan P W,Johnston J S.Environmentally correlated variation in 2C nuclear DNA C-content measurements in Helianthus annuus L.[J].Annals of Botany,1998,82(Supplement A):95-98.
29. Leitch I J,Soltis D E,Soltis P S,et al.Evolution of DNA amounts across land plants(Embryophyta)[J].Annals of Botany,2005,95(1):207-217.
30. Figar R B.Proleptic branch initiation in Michelia and Magnolia subgenus Yulania provides basis for combinations in subfamily Magnolioideae[A].//Liu Y H.Proceedings of the international symposium on the family Magnoliaceae[C].Beijing:Science Press,2000:14-25.
31. 王亚玲,崔铁成,张寿洲.木兰科植物系统学研究进展[J].西北林学院学报,2003,18(2):22-28. Wang Y L,Cui T C,Zhang S Z.The Studying Progress of Classification in Magnoliaceae[J].Journal of Northwest Forestry University,2003,18(2):22-28.

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