白木香结香过程中阻隔层的形成及其特性研究

doi:10.7525/j.issn.1673-5102.2016.05.010

植物研究 ›› 2016, Vol. 36 ›› Issue (5): 697-704.doi: 10.7525/j.issn.1673-5102.2016.05.010

白木香结香过程中阻隔层的形成及其特性研究

刘培卫^1,2, 杨云^1,2, 张玉秀^1,2, 杨勇^1,2, 刘洋洋^1,2

1. 中国医学科学院北京协和医学院药用植物研究所海南分所, 海口 570311;
2. 海南省南药资源保护与开发重点实验室, 海口 570311

收稿日期:2016-06-02 出版日期:2016-09-15 发布日期:2016-09-27
作者简介:刘培卫(1983-),男,助理研究员,主要从事南药植物结构和活性成分研究。
基金资助:
海南省自然科学基金（314183）；北京协和医学院协和青年科研基金（33320140078）；中医药行业科研专项（201407005）；海南省中药现代化项目（2015ZY13）

Barrier Zone Formation in Agarwood Formation of Aquilaria sinensis

LIU Pei-Wei^1,2, YANG Yun^1,2, ZHANG Yu-Xiu^1,2, YANG Yong^1,2, LIU Yang-Yang^1,2

1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311;
2. Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch, Haikou 570311

Received:2016-06-02 Online:2016-09-15 Published:2016-09-27
Supported by:
Natural Science Foundation of Hainan Province(314183);PUMC Youth Fund and the Fundamental Research Funds for the Central Universities(33320140078);Traditional Chinese Medicine Industry Special Project(201407005);Modernization of Traditional Chinese Medicine of Hainan Province(2015ZY13)

摘要/Abstract

摘要： 分别采用滑走切片、组织化学染色、石蜡切片技术、扫描电镜技术和分光光度法等，对白木香通体结香过程中阻隔层的形成过程、解剖结构等特性进行了研究。结果显示：木间韧皮部内的薄壁细胞在伤害刺激下脱分化、分裂产生阻隔层；阻隔层位于沉香层和过渡层之间，其厚度变化较大，由薄壁细胞群和阻隔层木质部组成；阻隔层中含有少量的淀粉和较多的可溶性总糖。上述研究结果为消除或延迟阻隔层的产生提供参考，为进一步优化通体结香技术提供帮助。

关键词: 白木香, 木间韧皮部, 阻隔层, 通体结香技术

Abstract: With the sliding section, histochemical staining, paraffin section, scanning electricity microscope and spectrophotometry, we studied the anatomical structure and histochemcial characteristics of barrier zone. The barrier zone was arisen from interxylary phloem. Barrier zone in different thickness located between agarwood zone and transition zone. The barrier zone was formed of abnormal parenchyma and xylem. Barrier zone contained a small amount of starch and more total sugar. Our results might be used to eliminate and delay the barrier zone, and improve whole-tree agarwood-inducing technique.

Key words: Aquilaria sinensis, interxylary phloem, barrier zone, whole-tree agarwood-inducing technique

中图分类号:

R282

刘培卫, 杨云, 张玉秀, 杨勇, 刘洋洋. 白木香结香过程中阻隔层的形成及其特性研究[J]. 植物研究, 2016, 36(5): 697-704.

LIU Pei-Wei, YANG Yun, ZHANG Yu-Xiu, YANG Yong, LIU Yang-Yang. Barrier Zone Formation in Agarwood Formation of Aquilaria sinensis[J]. Bulletin of Botanical Research, 2016, 36(5): 697-704.

参考文献

1. Liu Y Y,Chen H Q,Yang Y,et al.Whole-tree agarwood-inducing technique:an efficient novel technique for producing high-quality agarwood in cultivated Aquilaria sinensis trees[J].Molecules,2013,18(3):3086-3106.
2. 国家药典委员会.中华人民共和国药典2015年版(一部)[K].北京:中国医药科技出版社,2015:185.
3. Jung D.The cultural biography of agarwood-perfumery in eastern Asia and the Asian neighbourhood[J].Journal of the Royal Asiatic Society,2013,23(1):103-125.
4. 吕芳华,高维新.沉香贸易现状及发展前景研究[J].现代商贸工业,2013,25(6):65-66.Lü F H,Gao W X.Trade situation and development prospect on Aquilaria agallocha Roxb.[J].Modern Business Trade Industry,2013(06):65-66.
5. 张兴丽.伤害诱导的白木香防御反应与沉香形成的关系研究[D].北京:北京林业大学,2013.Zhang X L.Studies on Relationships between Wound-induced Defense response and Agarwood formation in Aquilaria sinensis[D].Beijing:Beijing Forestry University,2013.
6. CITES.Amendments to appendices Ⅰ,Ⅱ and Ⅲ of cites[C].//Proceedings of the thirteenth meeting of the conference of the parties.Bangkok,Thailand,2004.
7. 黄俊卿,魏建和,张争,等.沉香结香方法的历史记载、现代研究及通体结香技术[J].中国中药杂志,2013,38(3):302-306.Huang J Q,Wei J H,Zhang Z,et al.Historical records and modern studies on agarwood production method and overall agarwood production method[J].China Journal of Chinese Materia Medica,2013,38(3):302-306.
8. 张玉秀,刘培卫.木间韧皮部的定义、分布、发育和生理功能[J].湖北农业科学,2015,54(15):3589-3592.Zhang Y X,Liu P W.Intexylary phloem:definition,distribution,development and function[J].Hubei Agricultural Sciences,2015,54(15):3589-3592.
9. Carlquist S.Interxylary phloem:diversity and functions[J].Brittonia,2013,65(4):477-495.
10. Rao K R,Dayal R.The secondary xylem of Aquilaria agallocha(Thymelaeaceae) and the formation of ‘agar’[J].IAWA Journal,1992,13(2):163-172.
11. Shigo A L.Compartmentalization of decay in trees[J].Scientific American,1985,252(4):96-103.
12. Shigo A L.Compartmentalization:a conceptual framework for understanding how trees grow and defend themselves[J].Annual Review of Phytopathology,1984,22:189-214.
13. Blanchette R A,Biggs A R.Defense mechanisms of woody plants against fungi[M].Berlin Heidelberg:Springer,1992:1-12.
14. 张争,杨云,魏建和,等.白木香结香机制研究进展及其防御反应诱导结香假说[J].中草药,2010,41(1):156-159.Zhang Z,Yang Y,Wei J H,et al.Advances in studies on mechanism of agarwood formation in Aquilaria sinensis and its hypothesis of agarwood formation induced by defense response[J].Chinese Traditional and Herbal Drugs,2010,41(1):156-159.
15. Rioux D,Ouellette G B.Barrier zone formation in host and nonhost trees inoculated with Ophiostoma ulmi.Ⅱ.Ultrastructure[J].Canadian Journal of Botany,1991,69(9):2074-2083.
16. Smith K T.Compartmentalization today[J].Arboricultural Journal:The International Journal of Urban Forestry,2006,29(3):173-184.

白木香结香过程中阻隔层的形成及其特性研究

Barrier Zone Formation in Agarwood Formation of Aquilaria sinensis

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