红松多酚物质的提取工艺及其抗炎活性初步研究

doi:10.7525/j.issn.1673-5102.2016.04.021

植物研究 ›› 2016, Vol. 36 ›› Issue (4): 634-640.doi: 10.7525/j.issn.1673-5102.2016.04.021

• 研究简报 • 上一篇

红松多酚物质的提取工艺及其抗炎活性初步研究

祖元刚^1,2, 胡艳^1,2, 姜守刚^1,2

1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
2. 生物资源生态利用国家地方联合工程实验室, 哈尔滨 150040

收稿日期:2016-02-27 出版日期:2016-07-15 发布日期:2016-06-15
通讯作者: 姜守刚
作者简介:祖元刚(1954-),男,教授,博士,主要从事植物学的研究。
基金资助:
十二五农村领域国家科技支撑计划;杜仲和喜树珍贵材用和药用林定向培育关键技术研究与示范;水溶性喜树碱抗肿瘤活性研究(2012BAD21B0501-11);中央高校基本科研业务费专项资金项目;纳米紫杉醇制备及临床前药学评价(DL13CA11)

Extraction and Anti-inflammatory of Korean Pine Polyphenol

ZU Yuan-Gang^1,2, HU Yan^1,2, JIANG Shou-Gang^1,2

1. Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040;
2. State Engineering Laboratory of Bio-Resources Eco-Utilization, Northeast Forestry University, Harbin 150040

Received:2016-02-27 Online:2016-07-15 Published:2016-06-15
Supported by:
The Twelfth Five-Year National Science and technology supportprogram in rural areas;The key technology research and demonstration of the definite cultivation of eucommia ulmoides and camptotheca acuminate precious ingredients and medicinal forest(2012BAD21B0501-11);The Fundamental Research Funds for the Central Universities;Preparation and preclinical medicine evaluation of paclitaxelnanoparticles(DL13CA11)

摘要/Abstract

摘要： 选取超声波提取法对红松树皮中多酚类物质的提取工艺进行研究,以多酚提取率作为指标,采用单因素试验及正交试验,研究了物料比、乙醇浓度、提取时间、提取温度对红松多酚提取的影响。结果表明,超声波提取的最佳工艺条件是:物料比(g·mL^-1)1:30,乙醇浓度60%,提取时间2.0 h,提取温度80℃。本文也对红松多酚的抗炎作用进行了研究,并与杨树多酚、蓝莓多酚进行了比较,结果表明,在0.01~100 μg·mL^-1范围内,3种多酚都有一定的抗炎作用,且抗炎作用大小顺序为:蓝莓多酚> 红松多酚> 杨树多酚。

关键词: 红松多酚, 超声波提取, 抗炎活性, 杨树多酚, 蓝莓多酚

Abstract: The ultrasonic extraction process was used to extract polyphenols in Korean pine bark, with the polyphenol yield as index. The optimum conditions were obtained through single factor test and orthogonal test with 4 factors of material ratio, ethanol concentration, extract time and extract temperature. The optimal ultrasonic extract conditions were material ratio(g·mL^-1) of 1:30, ethanol concentration of 60%, extract time of 2.0 h, and extract temperature of 80℃. For the anti-inflammatory effect of Korean pine polyphenol, polar polyphenol and blueberry polyphenol, in 0.01-100 μg·mL^-1, the anti-inflammatory effect order was:blueberry polyphenol> Korean pine polyphenol> polar polyphenol.

Key words: korean pine polyphenol, ultrasonic extract, anti-inflammatory, polar polyphenol, blueberry polyphenol

中图分类号:

S791.247

祖元刚, 胡艳, 姜守刚. 红松多酚物质的提取工艺及其抗炎活性初步研究[J]. 植物研究, 2016, 36(4): 634-640.

ZU Yuan-Gang, HU Yan, JIANG Shou-Gang. Extraction and Anti-inflammatory of Korean Pine Polyphenol[J]. Bulletin of Botanical Research, 2016, 36(4): 634-640.

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红松多酚物质的提取工艺及其抗炎活性初步研究

Extraction and Anti-inflammatory of Korean Pine Polyphenol

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