油茶柠檬酸合成酶(CS)基因的克隆和表达分析

doi:10.7525/j.issn.1673-5102.2016.04.011

摘要/Abstract

摘要： 采用RT-PCR技术从油茶中分离出一个柠檬酸合成酶基因,该基因的cDNA全长1416 bp,编码471个氨基酸,推导的蛋白分子量为52.74 kD,理论等电点(PI)为6.95。同源比对显示其与其他植物的CS蛋白序列高度同源,将该基因命名为CoCS(Gen Bank登录号:KU161147)。系统进化树分析表明油茶CoCS与杜鹃和葡萄的CS蛋白的亲缘关系较近。荧光定量PCR分析结果表明,油茶受到低磷胁迫后根系CoCS基因的表达受到低磷诱导,表达量呈现先升高后降低的趋势;不同油茶品种不同组织(根、茎、叶)中的CoCS基因在不用磷处理下的表达模式不同。

关键词: 油茶, 柠檬酸合成酶, 低磷, 基因克隆, 表达分析

Abstract: A citrate synthase(CS) gene were isolated from Camellia oleifera by RT-PCR. The full-length cDNA of the CS is 1416 bp in size, encodeding a deduced polypetide of 471 amino acids with estimated molecular weight of 52.74 kD and theoretical isoelectric point of 6.95. Homologous alignment showed that the deduced protein had high identities with the CS proteins of other plants, therefore the gene was named as CoCS(Genbank No.KU161147). By phylogenetic tree analysis, CoCS had close genetic relationships with Rhododendron micranthum and Vitis vinifera. By qRT-PCR, the expression of CoCS in root was induced by phosphate deficiency and increased at first and decreased subsequently. The expression patterns of CoCS were different among different tissues and different cultivars.

Key words: Camellia oleifera, citrate synthase, phosphate deficient, gene cloning, expression analysis

中图分类号:

S794.4

叶思诚, 姚小华, 王开良, 林萍, 龚洪恩, 卓仁英. 油茶柠檬酸合成酶(CS)基因的克隆和表达分析[J]. 植物研究, 2016, 36(4): 556-564.

YE Si-Cheng, YAO Xiao-Hua, WANG Kai-Liang, LIN Ping, GONG Hong-En, ZHUO Ren-Ying. Cloning and Expression Analysis of Citrate Synthase(CS) Gene in Camellia oleifera[J]. Bulletin of Botanical Research, 2016, 36(4): 556-564.

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