胡杨无性系幼苗响应盐胁迫的miRNA表达差异研究

doi:10.7525/j.issn.1673-5102.2015.06.008

摘要/Abstract

摘要： 植物miRNA在调控基因表达、细胞周期、生物体发育、抗逆等方面起重要作用。为研究胡杨(Populus euphratica Oliv.)的耐盐机制，以1年生胡杨无性系幼苗为材料，构建具有空间代表性的盐胁迫胡杨cDNA文库，利用二代测序技术测定NaCl胁迫下和正常培养条件下胡杨叶和根miRNA表达情况。结果表明，不同的miRNA之间表达量存在明显差异，表达丰度最高的miRNA有miR156、miR157、miR165、miR166和miR167等，合计占总表达量的90%以上。胡杨根部存在特异表达的miRNA，在整个耐盐调控机制中发挥着生理调节、分子调控和信号传导等极为重要的作用。盐处理样品中发现大量响应盐胁迫的miRNA，对这些转录因子进行靶基因预测和注释后，发现很多盐胁迫响应的miRNA与NAC和SPL等重要转录因子家族相关，与前人的结论一致，另外还发现许多miRNA的调控对象是ATP酶和激素响应因子。

关键词: 高通量测序, miRNAs, 胡杨, 盐胁迫

Abstract:

MicroRNAs(miRNAs) have important regulatory functions in plant growth, development, cell cycle, gene expression regulation and response to abiotic stress. For studying the resistance of Populus euphratica to salt, we used 1-year-old poplar cutting clones to construct four small cDNA libraries from P.euphratica plantlets. We used the high-throughput sequencing to identify differentially expressed miRNAs treated with or without salt(300 mmol·L^-1 NaCl, 7 days). The different miRNA had significantly different expression. More than 90% expression were appeared in miR156, miR157, miR165, miR166, and miR167. The miRNA specific expressed in root played extremely important role in physiological, molecular regulation and signal transduction. Especially, many miRNAs were found in response to salt stress, and their target genes were predicted and annotated. These miRNAs were related to NAC and SPL transcription factor family, and they regulated ATP enzyme andhormone response factor.

Key words: high-throughput sequencing, microRNA, Populus euphratica, salt stress

中图分类号:

司婧娜；周韬；徐放；薄文浩；邬荣领*. 胡杨无性系幼苗响应盐胁迫的miRNA表达差异研究[J]. 植物研究, 2015, 35(6): 836-842.

SI Jing-Na；ZHOU Tao；XU Fang；BO Wen-Hao；WU Rong-Ling*. Salt-responsive MicroRNAs in Populus euphratica by Deep Sequencing[J]. Bulletin of Botanical Research, 2015, 35(6): 836-842.

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