关键词: LSD-Like基因家族, 转录因子, OsLOL2基因, 表达模式分析, 非生物逆境胁迫

Abstract: A known LSD1-Like type zinc finger protein transcription factor OsLOL2 gene(LOC_Os12g41700) was cloned from the rice Longjing 11(Oryza sativa L. longjing11). By bioinformatics analysis, the length of the ORF of OsLOL2 gene is 519 bp, which encoded 172 amino acid residues, with 2 LSD1-Like zinc finger domain. The transformation of epidermal cells of onion by Gene gun shows OsLOL2 locates in the nucleus. By real-time quantitative PCR, the transcriptional level of OsLOL2 gene increased significantly in Longjing 11 roots and leaves induced under 30 mmol·L^-1 NaHCO₃, 80 mmol·L^-1 NaCl, and 10 mmol·L^-1 H₂O₂ stress condition. Overexpression of OsLOL2 gene in yeast showed resistance to saline growth. Under stress condition, by the germination rate analysis, the germination rate of transgenic Arabidopsis was higher than that of wild type under NaCl and H₂O₂ stress. The germination rate and resistance on germination late showed the growing of overexpression strain was better than WT on 1/2 MS plate which contained 100 mmol·L^-1 NaCl, 3 mmol·L^-1 NaHCO₃ and 2 mmol·L^-1 H₂O₂. OsLOL2 gene could improve resistance for plant salt alkaline, which was related to the ROS way of oxidative stress by salt-alkali stress. Our study can provide a theoretical basis for studying OsLOL2 gene involved in stress signal way and lay the foundation for creating of new rice with stress resistance.

Key words: LSD-Like gene family, transcription factor, OsLOL2 gene, expression pattern analysis, abiotic stress