关键词: 马铃薯, StSnRK2.2基因, 克隆, 生物信息学分析

Abstract: The full-length cDNA sequence of SnRK2.2 in Solanum tuberosum (longshu 3) was successfully cloned by PCR. The gene was named StSnRK2.2 and has been submitted to Genbank with the accession number (GI:404435144). The bioinformatic analysis showed that the StSnRK2.2 has a complete opening reading frame (ORF) sequence contains 1 020 bp, which encoding 339 amino acids. The predicted protein molecular weight is 38.33 kDa and the theoretical pI is 5.93. The secondary structure of StSnRK2.2 indicated that about 43.36% of amino acids formed into alpha helix, 16.22% in extended strand, 6.78% in beta turn and 33.63% in random coil. There are no obviously classical signal peptide and transmembrane domain. The subcellular analysis indicated that the protein might play roles in the cell nucleus. Polygenetic analysis showed that the sequence is very close to the SnRK2 member in tobacco, the similar coefficient is as high as 90.56%. According to the amino acids sequence analysis, as a Ser/Thr protein kinase, the protein contains thirteen potential serine phosphatase sites, four threonine sites and four tyrosine sites. The results suggested that the gene product of StSnRK2.2 might be an important protein kinase related to the drought/stresses tolerance of potato plant.

Key words: Solanum tuberosum, StSnRK2.2, cloning, bioinformatic analysis