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植物研究 ›› 2012, Vol. 32 ›› Issue (6): 685-688.doi: 10.7525/j.issn.1673-5102.2012.06.009

• 论文 • 上一篇    下一篇

毛脉酸模的组织培养及植株再生

吴柏艳1,2;谢彦梅1;王振月1*   

  1. 1.黑龙江中医药大学药学院,哈尔滨 150040;2.哈药集团三精制药股份有限公司,哈尔滨 150069
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-11-20 发布日期:2012-11-20
  • 通讯作者: 王振月
  • 基金资助:
     

Tissue Culture and Plant Regeneration of Rumex gmelini Turcz.

WU Bai-Yan;XIE Yan-Mei;WANG Zhen-Yue*   

  1. 1.College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040;2.Harbi Pharm. Group Sanchine Pharmaceutical Holding Co,Ltd,Harbin 150069
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-20 Published:2012-11-20
  • Contact: WANG Zhen-Yue
  • Supported by:
     

摘要: 以毛脉酸模下胚轴和幼苗根茎为外植体,以MS为基本培养基,与不同浓度比例的6-BA和2,4-D等植物生长调节剂进行毛脉酸模组织培养的研究。结果表明,毛脉酸模幼苗根茎是诱导丛生芽的最佳材料,诱导丛生芽产生的最佳培养基为MS+6-BA 3.0 mg·L-1+2,4-D 0.1 mg·L-1,诱导率为81.2%;生根培养基为无激素的MS培养基,生根率可达92%,在此条件下生根迅速,可获得完整植株;组培苗经过炼苗移栽后成活率可达80%。

关键词: 毛脉酸模, 组织培养, 植株再生

Abstract: With the hypocotyls and seedling rhizomes of Rumex gmelini Turcz. as explants, the tissue culture of R.gmelini was studied by adding different concentrations of 6-BA and 2,4-D into MS basic mediums. The results showed that the better materials for multiple shoot clumps induction were seedling rhizomes; the best medium for multiple shoot clumps induction was MS+6-BA 3.0 mg·L-1+2,4-D 0.1 mg·L-1, the induction rate was 81.2%; the rooting medium was MS basic mediums without hormones, the rooting rate could get 92%, and under this conditions the plant regeneration could be obtained rapidly. After transplantation, about 80% of these seedlings could survive.

Key words: Rumex gmelini Turcz, tissue culture, plant regeneration

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