欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2012, Vol. 32 ›› Issue (3): 326-330.doi: 10.7525/j.issn.1673-5102.2012.03.013

• 论文 • 上一篇    下一篇

植物激素对不同烟草遗传瘤诱导的研究

曲冠证1;郑唐春1;马麟2;赵学彩1;李爽1;李开隆1*   

  1. 1.东北林业大学林木遗传育种国家重点实验室,哈尔滨 150040;2.吉林省临江林业局,临江 134600
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 李开隆
  • 基金资助:
     

Effect of Phytohormone on Induction of Genetic Tumor in Different Tobaccos

QU Guan-Zheng;ZHENG Tang-Chun;MA Lin;ZHAO Xue-Cai;LI Shuang;LI Kai-Long*   

  1. 1.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040;2.Linjiang Forestry Bureau of Jilin Province,Linjiang 134600
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-05-20 Published:2012-05-20
  • Contact: LI Kai-Long
  • Supported by:
     

摘要: 通过观察烟草品种N.glaucaN.langsdorffii和杂种N.glauca×N.langsdorffii的形态差异,利用IAA、6-BA、ABA等不同激素处理杂种N.glauca×N.langsdorffii探究激素对于遗传瘤产生的影响。实验结果显示生长素能显著抑制实生幼苗的遗传瘤产生,而分裂素则明显促进遗传瘤的产生。本实验还首次从烟草N.glauca中诱导产生遗传瘤,这说明种间杂交并不是诱导产生遗传瘤的唯一因素。电镜扫描结果显示遗传瘤中的细胞分化很不规则,可能是由于细胞分化及细胞间协作失控引起的。

关键词: 烟草, 杂种N.glauca×, N.langsdorffii, 遗传瘤, 植物激素

Abstract: The morphology of N.glauca, N.langsdorffii and N.glauca×N.langsdorffii was detected, and the effect of phytohormone on hybrid genetic tumor formation was analyzed. The result showed that exogenous cytokinin (N6-benzyladenine, BA) enhanced the tumor formation on hybrid seedling, while auxin (indole-3-acetic acid, IAA) inhibited. It was the first report on genetic tumor formation from N.glauca, suggesting that genetic factors may control tumor formation independent of the specific interspecific cross. The SEM assay showed that the tumor surface cells arranged irregularly and we proposed that the irregular arrangment of tumor cells was due to the loss of normal interaction and coordination between cells.

Key words: Tobacco, hybrid N.glauca×, N.langsdorffii, genetic tumor, phytohormone

中图分类号: