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植物研究 ›› 2011, Vol. 31 ›› Issue (3): 371-376.doi: 10.7525/j.issn.1673-5102.2011.03.022

• 论文 • 上一篇    下一篇

响应面法优化酶解辅助提取翅果油树叶总黄酮

郭彩珍;薛丹;侯学敏;张直峰;闫桂琴*   

  1. 山西师范大学生命科学学院,生物多样性研究所,临汾 041004
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
  • 通讯作者: 闫桂琴
  • 基金资助:
     

Optimization of Enzyme-assisted Extraction of Total Flavonoids from Elaeagnus mollis Diels Leaves by Using Response Surface Methodology

GUO Cai-Zhen;XUE Dan;HOU Xue-Min;ZHANG Zhi-Feng;YAN Gui-Qin*   

  1. School of Life Science,Institute of Biodiversity,Shanxi Normal University,Linfen 041004
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
  • Contact: YAN Gui-Qin
  • Supported by:
     

摘要: 利用酶辅助法对翅果油树(Elaeagnus mollis Diels)叶中总黄酮的提取工艺进行优化。在单因子试验结果的基础上,采用响应面法(RSM)研究了酶用量、酶解时间、pH值和酶解温度对翅果油树叶总黄酮提取得率的影响。结果表明:纤维素酶具有较好的酶解能力,当酶用量为6.5 mg·g-1,酶解时间为1.5 h,pH4.7,温度为46℃时,总黄酮得率达到7.1%。

关键词: 翅果油树叶, 总黄酮, 酶解, 响应面

Abstract: The process of enzyme-assisted extraction of total flavonoids from the leaves of Elaeagnus mollis Diels was optimized. Based on the single-factor tests, the effects of enzyme amount, time of enzymatic hydrolysis, pH value and temperature of enzymatic hydrolysis on the extraction yields of total flavonoids from E.mollis Diels leaves were studied by response surface methodology (RSM). The results indicated that cellulase showed better ability of enzymatic hydrolysis. The optimum enzyme-assisted extraction process was: enzyme amount 6.5 mg·g-1, time 1.5 h, pH value 4.7 and temperature 46℃. The highest yield of total flavonoids was 7.1%.

Key words: Elaeagnus mollis leaves, total flavonoids, enzymatic hydrolysis, RSM

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