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植物研究 ›› 2010, Vol. 30 ›› Issue (5): 562-567.doi: 10.7525/j.issn.1673-5102.2010.05.008

• 论文 • 上一篇    下一篇

孝顺竹愈伤组织增殖培养基优化研究

袁金玲;岳晋军;吴晓丽;张朵;郭广平;顾小平*   

  1. 中国林业科学研究院亚热带林业研究所,富阳 311400
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-20 发布日期:2010-09-20
  • 通讯作者: 顾小平
  • 基金资助:
     

Medium Optimization for Callus Proliferation of Bambusa multiplex

YUAN Jin-Ling;YUE Jin-Jun;WU Xiao-Li;ZHANG Duo;GUO Guang-Ping;GU Xiao-Ping*   

  1. Research Institute of Subtropical Forestry,CAF,Fuyang 311400
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
  • Contact: GU Xiao-Ping
  • Supported by:
     

摘要: 为筛选适宜孝顺竹愈伤组织继代增殖培养基,控制褐变发生,提高再生体系效率,对培养基组成如5种基本培养基、6种有机添加物、7种糖类和5种大量元素等因子进行试验分析。结果表明:培养20 d,基本培养基以MS效果较好,愈伤组织增殖2.8倍,白至淡黄色,致密;有机添加物以1.0 g·L-1脯氨酸效果较显著,愈伤组织增殖3.64倍,淡黄色,致密均一;碳源以30 g·L-1麦芽糖效果较好,愈伤组织增殖2.96倍,白至淡黄色,致密。5种大量元素中NH4NO3对孝顺竹愈伤组织增殖的影响达到显著水平,以825 mg·L-1为较佳浓度,培养29 d愈伤组织增殖可达5倍以上,部分出现根分化;适宜孝顺竹愈伤组织培养的大量元素组合为:KNO3 475 mg·L-1+NH4NO3 825 mg·L-1+MgSO4·7H2O 185 mg·L-1+KH2PO4 340 mg·L-1+CaCl2·7H2O 440 mg·L-1

关键词: 孝顺竹, 愈伤组织, 增殖, 培养基, 优化

Abstract: An effective regeneration system is the pre-requisite for genetic transformation of bamboos. Medium components for callus proliferation of Bambusa multiplex were studied, 5 basal medium, 6 organic additives, 7 sugars, and 5 macronutrients were compared. The results showed that Murashige and Skoog (MS) basal medium was effective, callus increased 2.8 times at 20 days, white to yellowish, compact; 1.0 g·L-1 proline was suitable the organic additive, callus increased 3.64 times, yellowish, compact and even; 30 g·L-1 maltose was the optimal carbon source, callus increased 2.96 times, white to yellowish, even. Among five macronutrients, NH4NO3 has significant influence on callus proliferation (α=0.05) and its optimal concentration was 825 mg·L-1, callus could increase over 5 times at 29 days, with some rooting; KNO3 475 mg·L-1+NH4NO3 825 mg·L-1+MgSO4·7H2O 185 mg·L-1+KH2PO4 340 mg·L-1+CaCl2·7H2O 440 mg·L-1 is a suitable combination for callus proliferation.

Key words: Bambusa multiplex, callus, proliferation, medium, optimization

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