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植物研究 ›› 2008, Vol. 28 ›› Issue (2): 240-243.doi: 10.7525/j.issn.1673-5102.2008.02.023

• 论文 • 上一篇    下一篇

长春花(Catharanthus roseus)中吲哚类生物碱含量的比较

张 琳;祖元刚*;牛卉颖;张颜滨;孙志强   

  1. (东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-03-20 发布日期:2008-03-20
  • 通讯作者: 祖元刚
  • 基金资助:
     

Comparative Study on the Contents of Indole Alkaloids in Catharanthus roseus

ZHANG Lin;ZU Yuan-Gang*;NIU Hui-Ying;ZHANG Yan-Bin;SUN Zhi-Qiang   

  1. (Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-03-20 Published:2008-03-20
  • Contact: ZU Yuan-Gang
  • Supported by:
     

摘要: 建立了反相高效液相法测定长春花中吲哚类生物碱文多灵、长春质碱和阿玛碱含量的方法,色谱柱为HiQ sil C18色谱柱(250 mm×4.6 mm,5 μm);流动相为1%二乙胺水溶液(磷酸调pH=7.2)—甲醇—乙腈/2:1:1(V/V);流速为1 mL·min-1;检测波长为215 nm;柱温为40℃。并采用此方法对长春花根、茎、叶、花和种子以及不同产地的长春花中的这3种生物碱进行了检测,结果表明文多灵和长春质碱主要存在于在根、茎、叶、花中,阿玛碱主要存在于种子中;并且随着地理位置的北移,长春花中的文多灵和长春质碱的含量逐渐降低,温室中人为控制长春花中的栽培条件能提高二者的含量。

关键词: 长春花, 文多灵, 长春质碱, 阿玛碱, HPLC

Abstract: A RP-HPLC quantification method was established to determine the contents of three indole alkaloids-vindoline, catharanthine and ajmalicine in Catharanthus roseus. HiQ sil C18 column(250 mm×4.6 mm,5 μm) was used. The mobile phase was 1% diethylamine(pH=7.2)-methanol-acetonitrile/2:1:1(V/V). The detection wavelength was 215 nm. The flow rate was 1 mL·min-1. The column temperature was 40℃. Different parts of C. roseus were determined and the results showed that vindoline and catharanthine mainly existed in roots, stems, leaves and flowers, while ajmalicine mainly existed in seeds. C. roseus from different habitats were also determined by this method and the results showed that the contents of vindoline and catharanthine in south habitats samples were higher than those in north habitats. For the cultured C. roseus in greenhouse, both vindoline and catharanthine contents were enhanced greatly.

Key words: Catharanthus roseus, vindoline, catharanthine, ajmalicine, HPLC

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