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植物研究 ›› 2008, Vol. 28 ›› Issue (1): 79-81.doi: 10.7525/j.issn.1673-5102.2008.01.017

• 论文 • 上一篇    下一篇

桔梗花药培养初报

吴京姬;吴基日*;吴松权;严一字;王立平   

  1. 延边大学农学院,龙井 133400
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-01-20 发布日期:2008-01-20
  • 通讯作者: 吴基日
  • 基金资助:
     

Preliminary Study of Anther Culture of Platycodon grandiflorum

WU Jing-Ji;WU Ji-Ri*;WU Song-Quan;YAN Yi-Zi;WANG Li-Ping   

  1. Agricultural College of Yanbian University, Longjing 133400
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-20 Published:2008-01-20
  • Contact: WU Ji-Ri
  • Supported by:
     

摘要: 本试验把花粉发育至单核靠边期的桔梗花药接在6种不同组合的培养基上诱导愈伤组织,并把诱导的愈伤组织转接到分化培养基,试图得出单倍体植株。结果表明:诱导率最高的培养基组合是N6 + 2,4-D 0.2mg·L-1 + 6-BA1.0 mg·L-1,诱导率为89.6%;在N6+6-BA1.0 mg·L-1 + NAA0.5 mg·L-1的分化培养基上最高分化率可达58.4%;经根尖压片检查染色体数目结果,分化的绿苗中弱小的植株是单倍体植株。

关键词: 桔梗, 花药培养, 单倍体植株

Abstract: Pollen of Platycodon grandiflorum growing to monokaryotic stage was inoculated in six different culture medium and induced callus, the induced callus was transfered in differentiation culture, trying to get haploidy. The results showed that the culture medium of the highest induced rate of callus was N6 + 2,4-D 0.2 mg·L-1 + 6-BA1.0 mg·L-1 and induced rate was 89.6%, the highest differentiation rate was 58.4% in the culture of N6 + 6-BA1.0 mg·L-1 + NAA0.5 mg·L-1, puny green plant in differentiation was haploidy with the result of chromosome number of root.

Key words: Platycodon grandiflorum, anther culture, haploidy

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