关键词: 花楸腋芽, 组织培养, 快速繁殖

Abstract: In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA(2.5 mg·L^-1) and 2.4-D(0.5 mg·L^-1); the optimum medium for bud proliferation was MS+6-BA(1 600~2 100)mg·L^-1+NAA(0.140~0.230)mg·L^-1, 13 shoots per explant could be obtained at average;the shoots grew strongly on MS+6-BA0.2 mg·L^-1+IBA0.2 mg·L^-1; And 1/2MS+IBA0.3 mg·L^-1 was the best medium for shoot rooting,with the rooting rate at 90.20%,and the average root number of 5.96;Plantlets survival rate reached 95.55% when transplanted on the mixture(humus 3:peat soil 2:sand 1).

Key words: Sorbus pohuashanensis axillary bud, tissue culture, rapid propagation