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植物研究 ›› 2007, Vol. 27 ›› Issue (1): 89-93.doi: 10.7525/j.issn.1673-5102.2007.01.017

• 论文 • 上一篇    下一篇

中国春—山羊草2C二体附加系同中国春—偃麦草二体附加系杂交后代的细胞遗传学研究

刘凤歧;曲 敏;张延明;张雪亭;李集临*   

  1. (哈尔滨师范大学生物系,哈尔滨 150025)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-01-20 发布日期:2007-01-20
  • 通讯作者: 李集临
  • 基金资助:
     

Cytogenetic Studies on the Cross-progenies Between Chinese Spring-Agilops 2C Disomic Addition and Chinese Spring-Elytriga Disomic Addition

LIU Feng-Qi;QU Min;ZHANG Yan-Ming;ZHANG Xue-Ting;LI Ji-Lin*   

  1. (Department of Biology,Harbin Normal University,Harbin 150025)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-01-20 Published:2007-01-20
  • Contact: LI Ji-Lin
  • Supported by:
     

摘要: 对中国春—长穗偃麦草(E.elongata 2n=14EE)二体附加系(1E-7E)与中国春—柱穗山羊草(Ae.cylindrica 2n=28 CCDD)2C二体附加系杂交后代进行细胞遗传学研究。结果表明,杂交当代(1E-7E)的结实率最高为36.06%,最低为19.43%,平均为29.5%。F1自交结实率最高为62.09%,最低为30.92%,平均为39.64%。对照实验和方差分析表明,杀配子染色体对不同品系杂交的结实率影响有差异。杂交F1减数分裂观察,看到F1单价体数超过理论数值,后期出现大量的染色体片段,认为这种异常现象与杀配子染色体的作用有关。

关键词: 杀配子染色体, 附加系, 结实率, 减数分裂

Abstract: The cytogenetics of the cross-progenies between Chinese Spring-Agilops 2C disomic addition and Chinese Spring-Elytriga disomic addition were studied. The results showed that The highest seed-setting of crossing generation is 36.06%, the lowest is 19.43% and average is 29.5%. The highest seed-setting of F1-selfed is 62.09%, the lowest is 30.92% and average is 39.64%. the control experiment and analysis of variance showed that the gametocidal chromosome effected on the difference of seed-set rate of hybridization in different lines. After scrutinizing the meiosis of pollen mother cells (PMS) in F1 hybrids, the results were concluded: the number of univalents exceeded the expected and some trivalents and tetravalents appeared at the same time. These were mainly caused by the gametocidal chromosome 2C.

Key words: gametocidal chromosome, addition lines, rate of seed set, meiosis

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