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植物研究 ›› 2006, Vol. 26 ›› Issue (4): 486-489.doi: 10.7525/j.issn.1673-5102.2006.04.005

• 论文 • 上一篇    下一篇

鲜茶叶中表没食子儿茶素没食子酸酯的高效提取技术研究

高彦华;祖元刚*;杨 磊;王 磊;朱 磊;王延兵   

  1. 东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-07-20 发布日期:2006-07-20
  • 通讯作者: 祖元刚
  • 基金资助:
     

The highly effecitive methods for extracting EGCG from fresh tea

GAO Yan-Hua;ZU Yuan-Gang*;YANG Lei;WANG Lei;ZHU Lei;WANG Yan-Bing   

  1. Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-20 Published:2006-07-20
  • Contact: ZU Yuan-Gang
  • Supported by:
     

摘要: 采用鲜磨匀浆提取、负压空化混悬组合技术对新鲜老龄茶叶中的没食子酸酯(EGCG)进行了高效提取,并与干燥老龄茶叶的传统提取工艺进行了对比。结果表明:组合工艺的最佳提取条件为新鲜老龄茶叶高温杀青后,再经常温水匀浆萃取,固液比为1:10,匀浆萃取时间为1 min,滤渣再经负压空化混悬固液提取,固液比为1:10,空化时间为15 min,空化提取2次。新鲜老龄茶叶最佳组合工艺的EGCG提取量为502.85 mg,优于传统干燥老龄茶叶工艺。

关键词: 表没食子儿茶素没食子酸酯, 匀浆, 负压空化混悬, 提取

Abstract: EGCG was effectively extracted from homogenates of fresh old age tealeaves by pulping and negative pressure-cavitation homogenous solid-liquid phase combined extraction technology. The extracting craftwork was compared with the traditional extraction of the old age tealeaves. The results showed that the fresh old age tealeaves were boiled to kill the enzymes firstly, the optimum combination extracting craftwork was as followed:the homogenate extract times was 1 min at normal temperature, the ratio of liquid to material was 1:10, then the homogenate filter residue was extracted by negative pressure-cavitation homogenous solidliquid phase. The EGCG content of the combined extraction was 502.85 mg. The combined craftwork with the fresh old age tealeaves was superior traditional extraction with dry ones.

Key words: EGCG, pulping, negative pressure-cavitation homogenous solid-liquid phase, extraction

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