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植物研究 ›› 2006, Vol. 26 ›› Issue (2): 198-200.doi: 10.7525/j.issn.1673-5102.2006.02.016

• 论文 • 上一篇    下一篇

落叶松幼苗过氧化氢酶动力学的初步研究

杨 茜1;乔 辰2;孙 君2;白雪华2   

  1. 1.内蒙古医学院, 呼和浩特 010059 2.内蒙古农业大学, 呼和浩特 010019
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-03-20 发布日期:2006-03-20
  • 通讯作者: 杨 茜
  • 基金资助:
     

A preliminary study on dynamics of catalase from larch seedling

YANG Qian;QIAO Chen;SUN Jun;BAI Xue-Hua   

  1. 1. Inner Mongolia Medical College, Hohhot 010059 2. Inner Mongolia Agricultural University, Huhhot 010018
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-03-20 Published:2006-03-20
  • Contact: YANG Qian
  • Supported by:
     

摘要: 采用碘量法对4种落叶松幼苗的过氧化氢酶(CAT)动力学进行了研究。结果表明:在25℃、pH 7.0下,落叶松幼苗CAT活性为48.8~77.9 U·g-1 FW;Km值为8.3×10-2~2.38×10-1 mol·L-1;Vmax为0.071~0.200 μmol·min-1;CAT最适温度15~25℃,对温度的适应范围华北落叶松和兴安落叶松宽,长白落叶松和太白落叶松的窄;CAT最适pH值为7.0。太白落叶松对pH适应范围最窄,其可能是退化的物种。

关键词: 落叶松, 幼苗, 过氧化氢酶, 动力学

Abstract: A study on dynamics of the catalase(CAT) from the seedling of four species of larch were made by means of iodometry. The results showed that CAT activities (25℃, pH 7.0) of larch seedling is 48.8 ~ 77.9 U·g-1 FW;Km values 8.3×10-2~2.38×10-1 mol·L-1;Vmax 0.071~0.200 μmol·min-1; Optimum temperatures 15~25℃. From adaptive range of temperature, L. principis-rupprechtii and L. gmelini are wider, L. olgensis and L. chinensis are narrower. Optimum pH 7.0. Adaptive range of L. chinensis is the narrowest, it may be degenerate species.

Key words: Larix, seedling, catalase, dynamics

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