关键词: 甘草, 甘草酸含量, 超声波提取法, 高效液相色谱法(HPLC)

Abstract: Extracted Glycyrrhizic acid (GA) from Glycyrrhiza uralensis Fisch by Ultrasonic extraction method, the conditions with higher GA were 0.3% ammonia solution as solvent, ratio of liquid to solid as 50:1(mL/g), extraction time five hours. The HPLC analysis conditions were as follows, chromatographic column ODS, detecting wavelength 254 nm, room temperature, mobile phase CH 3OH/3% HOAc(V/V) which ratio was 75/25, flowing rate 1 mL/min,injected sample volume 10 μL. The result of percent recovery is 100.20%, the relative standard deviation is 1.77%. Ultrasonic extraction-HPLC is a fast detecting GA method with higher accuracy from Glycyrrhiza uralensis.

Key words: Glycyrrhiza uralensis Fisch, glycyrrhizic acid content, ultrasonic extraction, high performance liquid chromagraphy